{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:18:46Z","timestamp":1791328726701,"version":"4.3.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1980,4]]},"abstract":"<jats:p>The isolation and biochemical properties of a Saccharomyces cerevisiae mutant (acc1-167) defective in acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP forming), EC 6.4.1.2] activity are described. The mutant is deficient in de novo biosynthesis of long-chain fatty acids and specifically requires a saturated fatty acid of chain length 14-16 C atoms for growth. Fatty acid synthetase levels were normal, but the acetyl-CoA carboxylase specific activity of the purified enzyme was reduced to approximately 5% compared to wild-type yeast. Upon sodium dodecyl sulfate\/polyacrylamide gel electrophoresis the purified mutant enzyme migrated as a single band and was essentially indistinguishable from the wild-type enzyme. The study of acetyl-CoA carboxylase partial activities revealed that the biotin incorporation capacity and the transcarboxylase partial activity were unaffected whereas the biotin carboxylase component enzyme exhibited less than 10% of wild-type specific activity. This biotin carboxylase mutational deficiency could be ascribed to a more than 90% reduction of Vmax and to a comparable increase in the Km value for ATP, which was accompanied by an increased requirement for Mg2+. It is concluded that acc1-167 contains a structural gene mutation in the biotin carboxylase domain of acetyl-CoA carboxylase.<\/jats:p>","DOI":"10.1073\/pnas.77.4.1814","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T04:13:05Z","timestamp":1149048785000},"page":"1814-1817","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":33,"title":["Fatty acid-requiring mutant of Saccharomyces cerevisiae defective in acetyl-CoA carboxylase."],"prefix":"10.1073","volume":"77","author":[{"given":"R","family":"Roggenkamp","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Numa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Schweizer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1980,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.77.4.1814","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:22:02Z","timestamp":1649848922000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.77.4.1814"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1980,4]]}},"alternative-id":["10.1073\/pnas.77.4.1814"],"URL":"https:\/\/doi.org\/10.1073\/pnas.77.4.1814","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,4]]},"assertion":[{"value":"1980-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}