{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:24:19Z","timestamp":1781105059107,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1981,4]]},"abstract":"<jats:p>A spontaneously transformed clone was isolated from repeatedly passaged BALB\/c 3T3 cells. The transformed cells were rounded or slender and elongated, were randomly arranged in an overlapping pattern, grew to high cell density, and had a low requirement for serum. The rates of multiplication and DNA synthesis of the nontransformed and the transformed lines were reduced for several days by drastic reduction in the Mg2+ concentration of the medium, but the rate of DNA synthesis in the Mg2+-deprived cultured increased after 6--8 days, suggesting an adaptation of the cells or a change in local environment. When maintained in very low Mg2+ concentrations the transformed cells assumed the appearance and arrangement of nontransformed cells within 1 day. The rate of DNA synthesis in the transformed cultures in 1.0 mM Mg2+ was independent of serum concentration. After 3 days of Mg2+ deprivation, however, the rate of DNA synthesis became highly dependent on both serum concentration and population density, thus resembling the growth behavior of nontransformed cells. Neither deprivation of K+ or Ca2+ nor addition of dibutyryl cyclic AMP produced these effects. The Mg2+ contents of nontransformed and transformed cells in physiological concentrations of Mg2+ were similar and only slightly reduced by incubation for 4 days in Mg2+-deficient medium. In 1.0 mM Mg2+, the Ca2+ content of the nontransformed cells was approximately 3 times higher than that of the transformed cells. After incubation in Mg2+-deficient medium, the Ca2+ contents of both cells types increased; that of the transformed cells slightly exceeded that of the nontransformed cells in Mg2+-sufficient medium. The results show that Mg2+-deprived transformed cells closely resemble nontransformed cells in appearance, requirement for serum, response to cell population density, and Ca2+ content. They also show that these parameters can be regulated coordinately by Mg2+ and support the suggestion that a defect in regulation by Mg2+ is a basic feature of the malignant transformation.<\/jats:p>","DOI":"10.1073\/pnas.78.4.2350","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T04:41:54Z","timestamp":1149050514000},"page":"2350-2354","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":15,"title":["Restoration of normal appearance, growth behavior, and calcium content to transformed 3T3 cells by magnesium deprivation."],"prefix":"10.1073","volume":"78","author":[{"given":"H","family":"Rubin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Vidair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Sanui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1981,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.78.4.2350","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:40:24Z","timestamp":1649850024000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.78.4.2350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1981,4]]}},"alternative-id":["10.1073\/pnas.78.4.2350"],"URL":"https:\/\/doi.org\/10.1073\/pnas.78.4.2350","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,4]]},"assertion":[{"value":"1981-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}