{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:17:51Z","timestamp":1781108271386,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1982,3]]},"abstract":"<jats:p>Fluid transport across the retinal pigment epithelium (bullfrog) has been measured. These experiments were carried out by using a capacitance probe technique and a water-impermeable chamber that allowed the measurements to be made with an accuracy of 0.5-1.0 nl\/min. With identical Ringer's solution on both sides of the epithelium, and in the absence of a hydrostatic driving force, the direction of net fluid movement is from the retina to the choroid (absorption). The net transport rate, approximately 10 nl\/min (4.8 microliters\/cm2 . hr), is comparable to that observed in other amphibian epithelia. It is reduced to zero by the mitochondrial uncoupler 2,4-dinitrophenol but is relatively unaffected by ouabain, which inhibits the Na+\/K+-pump located on the apical membrane of this epithelium. A significant decrease in net fluid absorption was produced by dibutyryl cAMP and 3-isobutyl-1-methylxanthine (a potent phosphodiesterase inhibitor). This cAMP-dependent fluid transport may be an important mechanism for controlling the fluid volume in the subretinal space.<\/jats:p>","DOI":"10.1073\/pnas.79.6.2111","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T05:03:20Z","timestamp":1149051800000},"page":"2111-2115","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":65,"title":["Fluid transport across retinal pigment epithelium is inhibited by cyclic AMP."],"prefix":"10.1073","volume":"79","author":[{"given":"S S","family":"Miller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B A","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T E","family":"Machen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1982,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.79.6.2111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:59:55Z","timestamp":1649851195000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.79.6.2111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,3]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1982,3]]}},"alternative-id":["10.1073\/pnas.79.6.2111"],"URL":"https:\/\/doi.org\/10.1073\/pnas.79.6.2111","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,3]]},"assertion":[{"value":"1982-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}