{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:29:02Z","timestamp":1781105342435,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"18","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1983,9]]},"abstract":"<jats:p>We have investigated the expression of H-2 antigen and beta 2-microglobulin in recently established mouse trophoblast cell clones. These clones, derived from C57BL\/6 (H-2b) or BALB\/c (H-2d) strains, synthesized extra-embryonic endoderm- and trophectoderm-specific cytoskeletal proteins, termed \"Endo A\" and \"Endo B,\" and no detectable SSEA-1 embryonic antigen. Flow microfluorometry indicated that H-2 antigen expression on the cell surface of trophoblast cells was very low, corresponding to 2-5% of the amounts found on differentiated teratocarcinoma cells (12-1a) and BALB\/c 3T3 cells, respectively. Expression of beta 2-microglobulin was reduced to approximately equal to 14% of the amounts found on 12-la cells. Immunoprecipitation and polyacrylamide gel electrophoretic analysis indicated that the synthesis of H-2 antigen and beta 2-microglobulin in the trophoblast cells was lower than that found in normal spleen cells. In addition low, but unequal, levels of mRNA specific for H-2 antigen and beta 2-microglobulin were found in trophoblast cells by blot hybridization with cDNA probes. The low mRNA levels may be due to transcriptional control of the genes encoding H-2 antigen and beta 2-microglobulin.<\/jats:p>","DOI":"10.1073\/pnas.80.18.5597","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T05:13:58Z","timestamp":1149052438000},"page":"5597-5601","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":5,"title":["Control of H-2 antigen and beta 2-microglobulin gene expression in mouse trophoblast cell clones."],"prefix":"10.1073","volume":"80","author":[{"given":"K","family":"Tanaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Ozato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Jay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J R","family":"Parnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Ramanathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J G","family":"Seidman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K S","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Appella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1983,9]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.80.18.5597","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:41:38Z","timestamp":1649850098000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.80.18.5597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,9]]},"references-count":0,"journal-issue":{"issue":"18","published-print":{"date-parts":[[1983,9]]}},"alternative-id":["10.1073\/pnas.80.18.5597"],"URL":"https:\/\/doi.org\/10.1073\/pnas.80.18.5597","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,9]]},"assertion":[{"value":"1983-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}