{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:06:20Z","timestamp":1781107580313,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1984,4]]},"abstract":"<jats:p>When the retinal Schiff base in chymotryptically cleaved bacteriorhodopsin is reduced to a secondary retinylamine by prolonged exposure to 10% (wt\/vol) sodium cyanoborohydride, at pH 10, in the absence of light, \u224845% of the retinal is found linked to Lys-41 and 22% to Lys-40, and the remainder is scattered over various sites on the large chymotryptic fragment, including the physiological site at Lys-216. The retinal-binding site is destroyed or blocked by the reduction conditions, but the bacteriorhodopsin lattice remains intact. The results demonstrate that artifactual linkage to Lys-40\/41 is possible under special conditions. Under these conditions, the \u03b5-amino groups of Lys-40\/41 show an enhanced ability to form retinylidene linkages with the retinal released by the physiological linkage site at Lys-216, due to some combination of close proximity to the normal linkage site, and increased reactivity with respect to other lysine \u03b5-amino groups. The results are of interest for the characterization of the two newly discovered rhodopsin-like proteins, halorhodopsin and slow rhodopsin.<\/jats:p>","DOI":"10.1073\/pnas.81.8.2303","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T05:39:40Z","timestamp":1149053980000},"page":"2303-2307","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":5,"title":["Retinal migration during dark reduction of bacteriorhodopsin"],"prefix":"10.1073","volume":"81","author":[{"given":"Paul K.","family":"Wolber","sequence":"first","affiliation":[{"name":"Cardiovascular Research Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walther","family":"Stoeckenius","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1984,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.81.8.2303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:02:13Z","timestamp":1649851333000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.81.8.2303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,4]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1984,4]]}},"alternative-id":["10.1073\/pnas.81.8.2303"],"URL":"https:\/\/doi.org\/10.1073\/pnas.81.8.2303","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,4]]},"assertion":[{"value":"1984-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}