{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:29:32Z","timestamp":1781105372671,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1987,9]]},"abstract":"<jats:p>We have isolated the individual gamma-crystallins expressed in young human lenses and identified with which of the six known human gamma-crystallin genes they each correspond. We find that at least 90% of the gamma-crystallins synthesized in the young human lens are the products of genes gamma G3 and gamma G4. We demonstrate that gamma G4-crystallin undergoes a temperature-dependent phase separation, and we have measured the low-concentration branch of its coexistence curve (phase separation temperature vs. concentration) up to about 40 mg\/ml. By comparison, we found no evidence of gamma G3-crystallin phase separating, even at lower temperatures and higher concentrations. This is consistent with predictions based on sequence homology between human and rat gamma-crystallins. The implications of these findings for human inherited and senile cataracts are considered.<\/jats:p>","DOI":"10.1073\/pnas.84.17.6088","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:23:39Z","timestamp":1149056619000},"page":"6088-6092","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":40,"title":["Human lens gamma-crystallins: isolation, identification, and characterization of the expressed gene products."],"prefix":"10.1073","volume":"84","author":[{"given":"R J","family":"Siezen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J A","family":"Thomson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E D","family":"Kaplan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G B","family":"Benedek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1987,9]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.84.17.6088","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:33:01Z","timestamp":1649853181000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.84.17.6088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,9]]},"references-count":0,"journal-issue":{"issue":"17","published-print":{"date-parts":[[1987,9]]}},"alternative-id":["10.1073\/pnas.84.17.6088"],"URL":"https:\/\/doi.org\/10.1073\/pnas.84.17.6088","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,9]]},"assertion":[{"value":"1987-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}