{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:37:45Z","timestamp":1792571865487,"version":"4.5.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1987,12]]},"abstract":"<jats:p>Deposition of amyloid in pancreatic islets is a common feature in human type 2 diabetic subjects but because of its insolubility and low tissue concentrations, the structure of its monomer has not been determined. We describe a peptide, of calculated molecular mass 3905 Da, that was a major protein component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. After collagenase treatment, an extract containing 20-50% amyloid was solubilized by sonication into 70% formic acid and the peptide was purified by gel filtration followed by reverse-phase high-performance liquid chromatography. We term this peptide diabetes-associated peptide, as it was not detected in extracts of pancreas from any of six normal subjects. Diabetes-associated peptide contains 37 amino acids and is 46% identical to the sequences of rat and human calcitonin gene-related peptide, indicating that these peptides are related in evolution. Sequence identities with conserved residues of the insulin A chain were also seen in a 16-residue segment. On extraction, the islet amyloid is particulate and insoluble like the core particles of Alzheimer disease. Their monomers have similar molecular masses, each having a hydropathic region that can probably form beta-pleated sheets. The accumulation of amyloid, including diabetes-associated peptide, in islets may impair islet function in type 2 diabetes mellitus.<\/jats:p>","DOI":"10.1073\/pnas.84.23.8628","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:29:20Z","timestamp":1149056960000},"page":"8628-8632","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1055,"title":["Purification and characterization of a peptide from amyloid-rich pancreases of type 2 diabetic patients."],"prefix":"10.1073","volume":"84","author":[{"given":"G J","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A C","family":"Willis","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R C","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R B","family":"Sim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K B","family":"Reid","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1987,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.84.23.8628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:11:47Z","timestamp":1649851907000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.84.23.8628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,12]]},"references-count":0,"journal-issue":{"issue":"23","published-print":{"date-parts":[[1987,12]]}},"alternative-id":["10.1073\/pnas.84.23.8628"],"URL":"https:\/\/doi.org\/10.1073\/pnas.84.23.8628","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,12]]},"assertion":[{"value":"1987-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}