{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:41:20Z","timestamp":1781109680426,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1987,12]]},"abstract":"<jats:p>We show that a 36-base-pair-long upstream fragment from the soybean hsp17.3-B gene comprising two partly overlapping heat-shock element (HSE)-like sequences can confer heat inducibility to a reporter gene in transgenic tobacco. The heat-shock response does not display organ specificity and is not affected by light. Insertion of these HSE-like elements into the pea rbcS-3A 5' flanking fragment (position -410 to +15) either at position -410 (5' to the enhancer) or at position -49 (between the enhancer and the \"TATA\" box) renders the transcript level of the reporter gene light-inducible and organ-specific under heat-shock conditions. These results demonstrate the possibility of generating a unique pattern of expression (e.g., light-dependent and organ-specific heat-shock response) by artificial combination of appropriate cis-acting regulatory elements. Moreover, by using the HSE-like sequences as a weak heat-inducible enhancer in the chimeric regulatory regions we uncover the function of negative elements within the pea rbcS-3A upstream region. These negative elements are responsible for a repressed transcript level in roots as well as in dark-adapted leaves. Therefore, the upstream fragment containing two HSE-like elements can be considered a useful tool to test the function of other cis-acting elements.<\/jats:p>","DOI":"10.1073\/pnas.84.24.8986","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:30:09Z","timestamp":1149057009000},"page":"8986-8990","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["Artificial combination of two cis-regulatory elements generates a unique pattern of expression in transgenic plants."],"prefix":"10.1073","volume":"84","author":[{"given":"G","family":"Strittmatter","sequence":"first","affiliation":[{"name":"Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N H","family":"Chua","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1987,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.84.24.8986","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:24:21Z","timestamp":1649852661000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.84.24.8986"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,12]]},"references-count":0,"journal-issue":{"issue":"24","published-print":{"date-parts":[[1987,12]]}},"alternative-id":["10.1073\/pnas.84.24.8986"],"URL":"https:\/\/doi.org\/10.1073\/pnas.84.24.8986","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,12]]},"assertion":[{"value":"1987-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}