{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:50:30Z","timestamp":1790441430048,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1987,12]]},"abstract":"<jats:p>We report on investigations aimed at detecting mutated RAS genes in a variety of preleukemic disorders and leukemias of myeloid origin. DNA transfection analyses (tumorigenicity assay) and hybridization to mutation-specific oligonucleotide probes established NRAS mutations in codon 12 or 61 of 4\/9 acute myelocytic leukemias (AML) and three AML lines. Leukemic cells of another AML patient showed HRAS gene activation. By using a rapid and sensitive dot-blot screening procedure based on the combination of in vitro amplification of RAS-specific sequences and oligonucleotide hybridization we additionally screened 15 myelodysplastic syndromes, 26 Philadelphia chromosome-positive chronic myelocytic leukemias in chronic or acute phase, and 19 other chronic myeloproliferative disorders. A mutation within NRAS codon 12 could thus be demonstrated in a patient with idiopathic myelofibrosis and in another with chronic myelomonocytic leukemia. Moreover, mutated NRAS sequences were detected in lymphocytes, in granulocytes, as well as in monocytes\/macrophages of the latter case.<\/jats:p>","DOI":"10.1073\/pnas.84.24.9228","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:30:09Z","timestamp":1149057009000},"page":"9228-9232","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":158,"title":["RAS gene mutations in acute and chronic myelocytic leukemias, chronic myeloproliferative disorders, and myelodysplastic syndromes."],"prefix":"10.1073","volume":"84","author":[{"given":"J W","family":"Janssen","sequence":"first","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A C","family":"Steenvoorden","sequence":"additional","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Lyons","sequence":"additional","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Anger","sequence":"additional","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J U","family":"B\u00f6hlke","sequence":"additional","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Bos","sequence":"additional","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Seliger","sequence":"additional","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C R","family":"Bartram","sequence":"additional","affiliation":[{"name":"Department of Pediatrics II, University of Ulm, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1987,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.84.24.9228","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:24:23Z","timestamp":1649852663000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.84.24.9228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,12]]},"references-count":0,"journal-issue":{"issue":"24","published-print":{"date-parts":[[1987,12]]}},"alternative-id":["10.1073\/pnas.84.24.9228"],"URL":"https:\/\/doi.org\/10.1073\/pnas.84.24.9228","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,12]]},"assertion":[{"value":"1987-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}