{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:11:08Z","timestamp":1791205868260,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1988,7]]},"abstract":"<jats:p>The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polypeptide by UV-crosslinking analysis. \"Footprint\" and methylation-interference analyses have shown that purified NF-kappa B has a binding activity specific for the kappa light chain enhancer sequence. The purified factor activated in vitro transcription of the human immunodeficiency virus type I promoter by binding to an upstream NF-kappa B-binding site.<\/jats:p>","DOI":"10.1073\/pnas.85.13.4700","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:38:04Z","timestamp":1149057484000},"page":"4700-4704","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":189,"title":["Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro."],"prefix":"10.1073","volume":"85","author":[{"given":"K","family":"Kawakami","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Scheidereit","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R G","family":"Roeder","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1988,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.85.13.4700","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:59:04Z","timestamp":1649854744000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.85.13.4700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7]]},"references-count":0,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1988,7]]}},"alternative-id":["10.1073\/pnas.85.13.4700"],"URL":"https:\/\/doi.org\/10.1073\/pnas.85.13.4700","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,7]]},"assertion":[{"value":"1988-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}