{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:16:11Z","timestamp":1790648171669,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1988,10]]},"abstract":"<jats:p>The regulatory peptide called calcitonin gene-related peptide (CGRP) was detected by immunofluorescence in frog motor neurons and motor nerve terminals. In motor nerve terminals, CGRP-like immunoreactivity was found to be segregated within large dense-core vesicles. To determine whether exocytosis from acetylcholine-containing small synaptic vesicles and from CGRP-containing large dense-core vesicles can be independently stimulated, nerve-muscle preparations were exposed to alpha-latrotoxin. This toxin induced complete depletion of acetylcholine-containing small synaptic vesicles but did not induce a parallel depletion of CGRP-like immunoreactivity and of large dense-core vesicles. These effects were independent of the presence of extracellular Ca2+ and occurred both at room temperature and at low temperature (1-3 degrees C). These findings suggest that exocytosis from the two vesicle populations is mediated by distinct biochemical mechanisms, which might be differentially regulated by physiological stimuli.<\/jats:p>","DOI":"10.1073\/pnas.85.19.7366","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:43:52Z","timestamp":1149057832000},"page":"7366-7370","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":170,"title":["Differential effect of alpha-latrotoxin on exocytosis from small synaptic vesicles and from large dense-core vesicles containing calcitonin gene-related peptide at the frog neuromuscular junction."],"prefix":"10.1073","volume":"85","author":[{"given":"M","family":"Matteoli","sequence":"first","affiliation":[{"name":"Consiglio Nazionale delle Ricerce Center of Cytopharmacology, University of Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Haimann","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale delle Ricerce Center of Cytopharmacology, University of Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Torri-Tarelli","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale delle Ricerce Center of Cytopharmacology, University of Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"Polak","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale delle Ricerce Center of Cytopharmacology, University of Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Ceccarelli","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale delle Ricerce Center of Cytopharmacology, University of Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"De Camilli","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale delle Ricerce Center of Cytopharmacology, University of Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1988,10]]},"updated-by":[{"DOI":"10.1073\/pnas.86.4.1416","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[1989,2,1]],"date-time":"1989-02-01T00:00:00Z","timestamp":602294400000}}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.85.19.7366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:49:33Z","timestamp":1649854173000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.85.19.7366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,10]]},"references-count":0,"journal-issue":{"issue":"19","published-print":{"date-parts":[[1988,10]]}},"alternative-id":["10.1073\/pnas.85.19.7366"],"URL":"https:\/\/doi.org\/10.1073\/pnas.85.19.7366","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,10]]},"assertion":[{"value":"1988-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}