{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:36:29Z","timestamp":1790498189622,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"22","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1988,11]]},"abstract":"<jats:p>Purified and reconstituted sodium channels from rat brain have been photoaffinity labeled with a photoactivable derivative of the alpha-scorpion toxin V from Leiurus quinquestriatus (LqTx). A battery of sequence-specific antibodies has been used to determine which of the peptides produced by chemical and enzymatic cleavage of the photolabeled sodium-channel alpha subunit contain covalently attached LqTx. Nearly all of the covalently attached LqTx is found within homologous domain I. Two site-directed antisera, which recognize residues 317 to 335 and residues 382 to 400, respectively, specifically immunoprecipitate a 14-kDa peptide produced by CNBr digestion to which LqTx is covalently attached. It is proposed that a portion of the receptor site for alpha-scorpion toxins is formed by peptide segment(s) between amino acid residues 335 and 378 which is located in an extracellular loop between transmembrane helices S5 and S6 of homologous domain I of the sodium channel alpha subunit.<\/jats:p>","DOI":"10.1073\/pnas.85.22.8742","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:46:49Z","timestamp":1149058009000},"page":"8742-8746","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":96,"title":["Site of covalent attachment of alpha-scorpion toxin derivatives in domain I of the sodium channel alpha subunit."],"prefix":"10.1073","volume":"85","author":[{"given":"F J","family":"Tejedor","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W A","family":"Catterall","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1988,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.85.22.8742","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:45:21Z","timestamp":1649853921000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.85.22.8742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11]]},"references-count":0,"journal-issue":{"issue":"22","published-print":{"date-parts":[[1988,11]]}},"alternative-id":["10.1073\/pnas.85.22.8742"],"URL":"https:\/\/doi.org\/10.1073\/pnas.85.22.8742","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,11]]},"assertion":[{"value":"1988-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}