{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:39:40Z","timestamp":1781105980292,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1988,4]]},"abstract":"<jats:p>This study documents the curing of a congenitally acquired chronic viral infection and the acquisition of T-cell competence by a previously tolerant host. Infection of mice with lymphocytic choriomeningitis virus (LCMV) is a classic model of viral persistence and antigen-specific T-cell unresponsiveness. Mice infected at birth or in utero become lifelong carriers with no detectable virus-specific cytotoxic T lymphocyte (CTL) responses. This chronic infection can be eliminated by adoptive transfer of Lyt-2+ T cells from LCMV-immune mice. To determine whether these cured carriers were capable of generating their own LCMV-specific CTL response, mice congenic at the Thy-1 locus (Thy-1.1 and Thy-1.2) were used in the adoptive transfer experiments. Host-derived T-cell responses were checked after treating the cured carriers with a monoclonal antibody to deplete the immune donor T cells. Such cured carrier mice were able to generate a host-derived virus-specific CTL response and resisted a second LCMV challenge in the absence of any donor T cells. In addition, bone marrow cells from these cured carriers could functionally reconstitute irradiated mice. Thus this report demonstrates the acquisition of LCMV-specific T-cell competence by previously unresponsive carrier mice infected in utero. These results show that exposure to a virus even during embryonic life does not cause a permanent deletion of specific T cells. These findings are of significance to the understanding of tolerance mechanisms and have implications for the treatment of chronic viral infections.<\/jats:p>","DOI":"10.1073\/pnas.85.7.2265","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:51:57Z","timestamp":1149058317000},"page":"2265-2268","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":44,"title":["T-cell tolerance: exposure to virus in utero does not cause a permanent deletion of specific T cells."],"prefix":"10.1073","volume":"85","author":[{"given":"B D","family":"Jamieson","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, Los Angeles School of Medicine 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, Los Angeles School of Medicine 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1988,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.85.7.2265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:39:15Z","timestamp":1649853555000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.85.7.2265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,4]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1988,4]]}},"alternative-id":["10.1073\/pnas.85.7.2265"],"URL":"https:\/\/doi.org\/10.1073\/pnas.85.7.2265","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,4]]},"assertion":[{"value":"1988-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}