{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:02:14Z","timestamp":1789189334871,"version":"build-2803163510"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:p>[(Dihydroindenyl)oxy]alkanoic acid (DIOA) was recently introduced as a potent inhibitor of the K+Cl- cotransport system without side effects on other cation transport systems [Garay, R. P., Nazaret, C., Hannaert, P.A. &amp; Cragoe, E. J., Jr. (1988) Mol. Pharmacol. 33, 696-701]. In sickle cells, an abnormal activation of this K+Cl- cotransport system was proposed to be involved in cell K+ loss and dehydration. We found that DIOA inhibited the abnormal sickle cell K+ loss and specifically reduced sickle cell density upon stimulation of the net outward K+Cl- cotransport--i.e., low pH, hypoosmolarity, and activation by N-ethylmaleimide. DIOA opens another therapeutic approach to sickle cell disease by inhibiting cell dehydration, which favors HbS polymerization and reduces erythrocyte deformability.<\/jats:p>","DOI":"10.1073\/pnas.86.11.4273","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:56:42Z","timestamp":1149058602000},"page":"4273-4276","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":41,"title":["Inhibition of K+ efflux and dehydration of sickle cells by [(dihydroindenyl)oxy]alkanoic acid: an inhibitor of the K+ Cl- cotransport system."],"prefix":"10.1073","volume":"86","author":[{"given":"D","family":"Vitoux","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U91, Centre National de la Recherche Scientifique UA 607, H\u00f4pital H. Mondor, Cr\u00e9tiel, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Olivieri","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U91, Centre National de la Recherche Scientifique UA 607, H\u00f4pital H. Mondor, Cr\u00e9tiel, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R P","family":"Garay","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U91, Centre National de la Recherche Scientifique UA 607, H\u00f4pital H. Mondor, Cr\u00e9tiel, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"E J","family":"Cragoe","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U91, Centre National de la Recherche Scientifique UA 607, H\u00f4pital H. Mondor, Cr\u00e9tiel, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Galacteros","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U91, Centre National de la Recherche Scientifique UA 607, H\u00f4pital H. Mondor, Cr\u00e9tiel, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Beuzard","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U91, Centre National de la Recherche Scientifique UA 607, H\u00f4pital H. Mondor, Cr\u00e9tiel, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,6]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.11.4273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:58:30Z","timestamp":1649854710000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.11.4273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1989,6]]}},"alternative-id":["10.1073\/pnas.86.11.4273"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.11.4273","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6]]},"assertion":[{"value":"1989-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}