{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:55:32Z","timestamp":1782352532343,"version":"3.54.5"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:p>For isolation of the gene responsible for xeroderma pigmentosum (XP) complementation group A, plasmid pSV2gpt and genomic DNA from a mouse embryo were cotransfected into XP2OSSV cells, a group-A XP cell line. Two primary UV-resistant XP transfectants were isolated from about 1.6 X 10(5) pSV2gpt-transformed XP colonies. pSV2gpt and genomic DNA from the primary transfectants were again cotransfected into XP2OSSV cells and a secondary UV-resistant XP transfectant was obtained by screening about 4.8 X 10(5) pSV2gpt-transformed XP colonies. The secondary transfectant retained fewer mouse repetitive sequences. A mouse gene that complements the defect of XP2OSSV cells was cloned into an EMBL3 vector from the genome of a secondary transfectant. Transfections of the cloned DNA also conferred UV resistance on another group-A XP cell line but not on XP cell lines of group C, D, F, or G. Northern blot analysis of poly(A)+ RNA with a subfragment of cloned mouse DNA repair gene as the probe revealed that an approximately 1.0 kilobase mRNA was transcribed in the donor mouse embryo and secondary transfectant, and approximately 1.0- and approximately 1.3-kilobase mRNAs were transcribed in normal human cells, but none of these mRNAs was detected in three strains of group-A XP cells. These results suggest that the cloned DNA repair gene is specific for group-A XP and may be the mouse homologue of the group-A XP human gene.<\/jats:p>","DOI":"10.1073\/pnas.86.14.5512","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:59:30Z","timestamp":1149058770000},"page":"5512-5516","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":75,"title":["Molecular cloning of a mouse DNA repair gene that complements the defect of group-A xeroderma pigmentosum."],"prefix":"10.1073","volume":"86","author":[{"given":"K","family":"Tanaka","sequence":"first","affiliation":[{"name":"Institute for Molecular and Cellular Biology, Osaka University, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Satokata","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Biology, Osaka University, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Ogita","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Biology, Osaka University, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Uchida","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Biology, Osaka University, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Okada","sequence":"additional","affiliation":[{"name":"Institute for Molecular and Cellular Biology, Osaka University, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.14.5512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:44:55Z","timestamp":1649853895000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.14.5512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7]]},"references-count":0,"journal-issue":{"issue":"14","published-print":{"date-parts":[[1989,7]]}},"alternative-id":["10.1073\/pnas.86.14.5512"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.14.5512","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7]]},"assertion":[{"value":"1989-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}