{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T21:41:40Z","timestamp":1789940500929,"version":"4.0.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:p>Expression of human T-cell leukemia virus type I (HTLV-I) is not detectable by immunofluorescence analysis or RNA blot analysis in most fresh peripheral blood mononuclear cells of patients with adult T-cell leukemia or of asymptomatic HTLV-I carriers. However, in this work, mRNA for the HTLV-I tax1\/rex1 genes was detected in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and asymptomatic HTLV-I carriers by using reverse transcription followed by the polymerase chain reaction. By using fresh peripheral blood mononuclear cells, the expression of tax1\/rex1 mRNA was detected in five of the six adult T-cell leukemia patients and four of the eight HTLV-I carriers examined. The amounts of tax1\/rex1 mRNA detected corresponded to approximately 10(5) to 10(6) times less than that in the HTLV-I-infected MT-2 cell line. These results indicate that, in some individuals infected with HTLV-I, the provirus in circulating blood cells is transcribed in vivo. Thus the expression of viral antigens in circulating blood cells in vivo is suggested.<\/jats:p>","DOI":"10.1073\/pnas.86.14.5620","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:59:30Z","timestamp":1149058770000},"page":"5620-5624","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":214,"title":["Detection of mRNA for the tax1\/rex1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction."],"prefix":"10.1073","volume":"86","author":[{"given":"T","family":"Kinoshita","sequence":"first","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Shimoyama","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Tobinai","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Ito","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ito","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Tajima","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Shimotohno","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Sugimura","sequence":"additional","affiliation":[{"name":"Virology Division, National Cancer Center Research Institute, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.14.5620","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:44:05Z","timestamp":1649853845000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.14.5620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7]]},"references-count":0,"journal-issue":{"issue":"14","published-print":{"date-parts":[[1989,7]]}},"alternative-id":["10.1073\/pnas.86.14.5620"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.14.5620","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7]]},"assertion":[{"value":"1989-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}