{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T03:57:44Z","timestamp":1783569464451,"version":"3.55.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,8]]},"abstract":"<jats:p>A single-base deletion in the single-copy vasopressin gene is the cause of diabetes insipidus in the homozygous Brattleboro rat (di\/di). It results in the synthesis of an altered vasopressin precursor of which the axonal transport is blocked. Paradoxically, a small number of solitary hypothalamic neurons displays all the immunoreactivities of the wild-type vasopressin precursor (i.e., vasopressin, neurophysin, and a glycopeptide). In the present paper we provide evidence that these neurons have undergone a switch to a genuine heterozygous (di\/+) phenotype; i.e., they contain the immunoreactivities of both the wild-type and the mutated vasopressin precursors. In the neural lobe, glycopeptide fibers are also present, showing that axonal transport of the wild-type precursor is restored. Moreover, the number of neurons displaying this di\/+ phenotype increases markedly and in a linear way (from 0.1% up to 3% of the vasopressin cells) with age. These findings indicate that after mitotic division has ceased, genomic alterations occur in somatic neurons in vivo. The molecular event generating the di\/+ phenotype in the di\/di animal could involve a somatic intrachromosomal gene conversion between the homologous exons of the vasopressin and the related oxytocin genes.<\/jats:p>","DOI":"10.1073\/pnas.86.16.6417","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:01:59Z","timestamp":1149058919000},"page":"6417-6420","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":56,"title":["Age-related development of a heterozygous phenotype in solitary neurons of the homozygous Brattleboro rat."],"prefix":"10.1073","volume":"86","author":[{"given":"F","family":"van Leeuwen","sequence":"first","affiliation":[{"name":"Netherlands Institute for Brain Research, Amsterdam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"van der Beek","sequence":"additional","affiliation":[{"name":"Netherlands Institute for Brain Research, Amsterdam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Seger","sequence":"additional","affiliation":[{"name":"Netherlands Institute for Brain Research, Amsterdam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Burbach","sequence":"additional","affiliation":[{"name":"Netherlands Institute for Brain Research, Amsterdam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Ivell","sequence":"additional","affiliation":[{"name":"Netherlands Institute for Brain Research, Amsterdam."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,8]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.16.6417","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:47:42Z","timestamp":1649854062000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.16.6417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,8]]},"references-count":0,"journal-issue":{"issue":"16","published-print":{"date-parts":[[1989,8]]}},"alternative-id":["10.1073\/pnas.86.16.6417"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.16.6417","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,8]]},"assertion":[{"value":"1989-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}