{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:43:59Z","timestamp":1781106239561,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,9]]},"abstract":"<jats:p>We have used cell-free DNA replication to study the relationship between DNA replication and chromatin assembly. As others have reported, we find that DNA replication facilitates nucleosome assembly. We show here that replication-dependent nucleosome assembly occurs in at least two steps. The first step requires replicating DNA; the second step occurs after replication has been completed and is promoted by a nuclear extract. Consistent with this multistep model, we observe that the replicated simian virus 40 minichromosome is organized into a repeating array of DNA-protein particles that are structurally distinct from mature nucleosomes. These particles may be precursors in a pathway of nucleosome assembly since in the second, replication-independent step the nuclear extract converts this nascent chromatin into nucleosomes.<\/jats:p>","DOI":"10.1073\/pnas.86.17.6459","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:02:09Z","timestamp":1149058929000},"page":"6459-6463","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":30,"title":["Multistep pathway for replication-dependent nucleosome assembly."],"prefix":"10.1073","volume":"86","author":[{"given":"R","family":"Fotedar","sequence":"first","affiliation":[{"name":"Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"Roberts","sequence":"additional","affiliation":[{"name":"Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,9]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.17.6459","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:45:59Z","timestamp":1649853959000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.17.6459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,9]]},"references-count":0,"journal-issue":{"issue":"17","published-print":{"date-parts":[[1989,9]]}},"alternative-id":["10.1073\/pnas.86.17.6459"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.17.6459","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,9]]},"assertion":[{"value":"1989-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}