{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:58:27Z","timestamp":1788350307250,"version":"build-2803163510"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,12]]},"abstract":"<jats:p>We demonstrate that blockade of the lymphocyte voltage-gated K+ channel by charybdotoxin (CTX) inhibits lymphocyte mitogenesis. Charybdotoxin blocks conductance with a Ki of 0.3 nM and inhibits mitogen- and antigen-stimulated proliferation with a Ki of 0.5 nM. As opposed to the other blockers of the lymphocyte K+ channel, the inhibition of mitogenesis by CTX can be overcome selectively by exogenous recombinant interleukin 2 (IL-2); endogenous levels of IL-2 in the culture supernatants of stimulated cells are decreased by the presence of CTX. Our results suggest that the voltage-gated K+ channel is either directly or indirectly involved in IL-2 synthesis and\/or secretion.<\/jats:p>","DOI":"10.1073\/pnas.86.24.10171","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:10:20Z","timestamp":1149059420000},"page":"10171-10175","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":126,"title":["Charybdotoxin inhibits proliferation and interleukin 2 production in human peripheral blood lymphocytes."],"prefix":"10.1073","volume":"86","author":[{"given":"M","family":"Price","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S C","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Deutsch","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.24.10171","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:59:37Z","timestamp":1649854777000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.24.10171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,12]]},"references-count":0,"journal-issue":{"issue":"24","published-print":{"date-parts":[[1989,12]]}},"alternative-id":["10.1073\/pnas.86.24.10171"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.24.10171","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,12]]},"assertion":[{"value":"1989-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}