{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:52:24Z","timestamp":1781106744612,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,3]]},"abstract":"<jats:p>The polymerase chain reaction was used to amplify DNA surrounding the codon 12 region of the c-Ki-ras gene from C3H\/10T1\/2 cells and from a number of 3-methylcholanthrene (MCA)-transformed derivatives of these cells. Sequence analysis demonstrated that tumorigenic MCAC116\/39 cells, known by DNA-mediated transfection to contain an activated c-Ki-ras oncogene, had a G----T transversion in the first position of codon 12 of this gene, resulting in a Gly12----Cys mutation. A combination of polymerase chain-reaction amplification and oligonucleotide hybridization demonstrated that three additional tumorigenic MCA transformants of C3H\/10T1\/2 cells had an identical mutation in the c-Ki-ras gene. In contrast, this mutation was not present in an MCA-induced C3H\/10T 1\/2 transformant that was not tumorigenic. The molecular specificity of this MCA-induced mutation resulting in C3H\/10T1\/2 tumorigenic transformants should provide an excellent system in which to study the roles of transcription, replication, repair, and exogenous factors in the establishment and expression of transformation and tumorigenicity.<\/jats:p>","DOI":"10.1073\/pnas.86.5.1608","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:13:59Z","timestamp":1149059639000},"page":"1608-1611","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":8,"title":["Tumorigenic methylcholanthrene transformants of C3H\/10T1\/2 cells have a common nucleotide alteration in the c-Ki-ras gene."],"prefix":"10.1073","volume":"86","author":[{"given":"A C","family":"Chen","sequence":"first","affiliation":[{"name":"Molecular Biology Institute, University of California, Los Angeles Center for the Health Sciences 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H R","family":"Herschman","sequence":"additional","affiliation":[{"name":"Molecular Biology Institute, University of California, Los Angeles Center for the Health Sciences 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.5.1608","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:44:37Z","timestamp":1649853877000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.5.1608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,3]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1989,3]]}},"alternative-id":["10.1073\/pnas.86.5.1608"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.5.1608","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,3]]},"assertion":[{"value":"1989-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}