{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:20:18Z","timestamp":1791206418607,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:p>Cell lines derived from Kaposi sarcoma lesions of patients with AIDS (AIDS-KS cells) produce several cytokines, including an endothelial cell growth factor, interleukin 1 beta, and basic fibroblast growth factor. Since exposure to human immunodeficiency virus increases interleukin 6 (IL-6) production in monocytes and endothelial cells produce IL-6, we examined IL-6 expression and response in AIDS-KS cell lines and IL-6 expression in AIDS Kaposi sarcoma tissue. The AIDS-KS cell lines (N521J and EKS3) secreted large amounts of immunoreactive and biologically active IL-6. We found both IL-6 and IL-6 receptor (IL-6-R) RNA by slot blot hybridization analysis of AIDS-KS cells. The IL-6-R was functional, as [3H]thymidine incorporation by AIDS-KS cells increased significantly after exposure to human recombinant IL-6 (hrIL-6) at greater than 10 units\/ml. When AIDS-KS cells (EKS3) were exposed to IL-6 antisense oligonucleotide, cellular proliferation decreased by nearly two-thirds, with a corresponding decrease in the production of IL-6. The decrease from IL-6 antisense in AIDS-KS cell proliferation was reversed by the addition of hrIL-6. We confirmed that AIDS-KS cells produced IL-6 in vivo by preparing RNA and tissue sections from involved and uninvolved skin from a patient with AIDS Kaposi sarcoma. We detected immunoreactive IL-6 in the involved tumor areas and to a lesser extent in the surrounding normal epidermis. Slot blot hybridization showed a great excess of IL-6 and IL-6-R RNA in involved skin compared to uninvolved skin. These results show that both IL-6 and IL-6-R are produced by AIDS-KS cells and that IL-6 is required for optimal AIDS-KS cell proliferation, and they suggest that IL-6 is an autocrine growth factor for AIDS-KS cells.<\/jats:p>","DOI":"10.1073\/pnas.87.11.4068","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:20:27Z","timestamp":1149060027000},"page":"4068-4072","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":339,"title":["AIDS Kaposi sarcoma-derived cells produce and respond to interleukin 6."],"prefix":"10.1073","volume":"87","author":[{"given":"S A","family":"Miles","sequence":"first","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A R","family":"Rezai","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J F","family":"Salazar-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Vander Meyden","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R H","family":"Stevens","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D M","family":"Logan","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R T","family":"Mitsuyasu","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Taga","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Hirano","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Division of Hematology-Oncology, University of California School of Medicine, Los Angeles 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1990,6]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.87.11.4068","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:50:32Z","timestamp":1649854232000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.87.11.4068"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1073\/pnas.87.11.4068"],"URL":"https:\/\/doi.org\/10.1073\/pnas.87.11.4068","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]},"assertion":[{"value":"1990-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}