{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:25:47Z","timestamp":1792797947639,"version":"4.5.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:p>Osteopetrotic (op\/op) mutant mice suffer from congenital osteopetrosis due to a severe deficiency of osteoclasts. Furthermore, the total number of mononuclear phagocytes is extremely low in affected mice. Serum, 11 tissues, and different cell and organ conditioned media from op\/op mice were shown to be devoid of biologically active colony-stimulating factor 1 (CSF-1), whereas all of these preparations from littermate control +\/+ and +\/op mice contained the growth factor. The deficiency was specific for CSF-1 in that serum or conditioned media from op\/op mice possessed elevated levels of at least three other macrophage growth factors. Partial correction of the op\/op defect was observed following intraperitoneal implantation of diffusion chambers containing L929 cells, which in culture produce CSF-1 as their sole macrophage growth factor. No rearrangement of the CSF-1 gene in op\/op mice was detected by Southern analysis. However, in contrast to control lung fibroblasts, which contained 4.6- and 2.3-kilobase CSF-1 mRNAs, only the 4.6-kilobase species was detected in op\/op cells. An alteration in the CSF-1 gene is strongly implicated as the primary defect in op\/op mice because they do not contain detectable CSF-1, their defect is correctable by administration of CSF-1, the op locus and the CSF-1 gene map within the same region of mouse chromosome 3, their CSF-1 mRNA biosynthesis is altered, and the op\/op phenotype is consistent with the phenotype expected in a CSF-1 deficient mouse.<\/jats:p>","DOI":"10.1073\/pnas.87.12.4828","type":"journal-article","created":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T21:05:57Z","timestamp":1149195957000},"page":"4828-4832","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":828,"title":["Total absence of colony-stimulating factor 1 in the macrophage-deficient osteopetrotic (op\/op) mouse."],"prefix":"10.1073","volume":"87","author":[{"given":"W","family":"Wiktor-Jedrzejczak","sequence":"first","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Bartocci","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"A W","family":"Ferrante","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Ahmed-Ansari","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K W","family":"Sell","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J W","family":"Pollard","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E R","family":"Stanley","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1990,6]]},"updated-by":[{"DOI":"10.1073\/pnas.88.13.5937d","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[1991,7,1]],"date-time":"1991-07-01T00:00:00Z","timestamp":678326400000}}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.87.12.4828","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:48:32Z","timestamp":1649854112000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.87.12.4828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1073\/pnas.87.12.4828"],"URL":"https:\/\/doi.org\/10.1073\/pnas.87.12.4828","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]},"assertion":[{"value":"1990-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}