{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:32:49Z","timestamp":1781109169833,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:p>Entamoeba histolytica causes amebic dysentery and amebic liver abscess, major causes of morbidity and mortality worldwide. We have used differential hybridization screening to isolate an E. histolytica-specific cDNA clone. The cDNA was found to encode a serine-rich E. histolytica protein (SREHP) containing multiple tandem repeats. The structural motif of SREHP resembles some of the repetitive antigens of malarial species, especially the circumsporozoite proteins. A recombinant trpE fusion protein containing the tandem repeats of SREHP was recognized by immune serum from a patient with amebiasis, demonstrating that SREHP is a naturally immunogenic protein. An antiserum raised against the recombinant fusion protein specifically bound to two distinct bands with apparent molecular masses of 46 and 52 kDa in a crude preparation of E. histolytica trophozoite membranes. This antiserum also inhibited E. histolytica trophozoite adhesion to Chinese hamster ovary cells in vitro. The ability to isolate E. histolytica-specific genes, and to express those genes in Escherichia coli, may be important in studying the molecular basis of E. histolytica pathogenesis and for the future development of vaccines.<\/jats:p>","DOI":"10.1073\/pnas.87.13.4976","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:22:10Z","timestamp":1149060130000},"page":"4976-4980","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":84,"title":["Cloning and expression of a membrane antigen of Entamoeba histolytica possessing multiple tandem repeats."],"prefix":"10.1073","volume":"87","author":[{"suffix":"Jr","given":"S L","family":"Stanley","sequence":"first","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Becker","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Kunz-Jenkins","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Foster","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1990,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.87.13.4976","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:58:02Z","timestamp":1649854682000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.87.13.4976"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,7]]},"references-count":0,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1990,7]]}},"alternative-id":["10.1073\/pnas.87.13.4976"],"URL":"https:\/\/doi.org\/10.1073\/pnas.87.13.4976","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,7]]},"assertion":[{"value":"1990-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}