{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:02:56Z","timestamp":1791169376822,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"15","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:p>To address the issue of whether regulatory feedback exists between the electrical activity of a neuron and ion-channel density, we investigated the effect of Na(+)-channel activators (scorpion alpha toxin, batrachotoxin, and veratridine) on the density of Na+ channels in fetal rat brain neurons in vitro. A partial but rapid (t1\/2, 15 min) disappearance of surface Na+ channels was observed as measured by a decrease in the specific binding of [3H]saxitoxin and 125I-labeled scorpion beta toxin and a decrease in specific 22Na+ uptake. Moreover, the increase in the number of Na+ channels that normally occurs during neuronal maturation in vitro was inhibited by chronic channel activator treatment. The induced disappearance of Na+ channels was abolished by tetrodotoxin, was found to be dependent on the external Na+ concentration, and was prevented when either choline (a nonpermeant ion) or Li+ (a permeant ion) was substituted for Na+. Amphotericin B, a Na+ ionophore, and monensin were able to mimick the effect of Na(+)-channel activators, while a KCl depolarization failed to do this. This feedback regulation seems to be a neuronal property since Na(+)-channel density in cultured astrocytes was not affected by channel activator treatment or by amphotericin B. The present evidence suggests that an increase in intracellular Na+ concentration, whether elicited by Na(+)-channel activators or mediated by a Na+ ionophore, can induce a decrease in surface Na+ channels and therefore is involved in down-regulation of Na(+)-channel density in fetal rat brain neurons in vitro.<\/jats:p>","DOI":"10.1073\/pnas.87.15.5907","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:24:19Z","timestamp":1149060259000},"page":"5907-5911","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":58,"title":["Down-regulation of voltage-dependent sodium channels initiated by sodium influx in developing neurons."],"prefix":"10.1073","volume":"87","author":[{"given":"B","family":"Dargent","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie, Centre National de la Recherche Scientifique UA 1179, Facult\u00e9 de M\u00e9decine-Secteur Nord, Marseille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Couraud","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie, Centre National de la Recherche Scientifique UA 1179, Facult\u00e9 de M\u00e9decine-Secteur Nord, Marseille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1990,8]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.87.15.5907","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:21:47Z","timestamp":1649856107000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.87.15.5907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8]]},"references-count":0,"journal-issue":{"issue":"15","published-print":{"date-parts":[[1990,8]]}},"alternative-id":["10.1073\/pnas.87.15.5907"],"URL":"https:\/\/doi.org\/10.1073\/pnas.87.15.5907","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,8]]},"assertion":[{"value":"1990-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}