{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T21:50:15Z","timestamp":1788558615768,"version":"build-2803163510"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:p>We have developed a reliable method for the direct resolution of haplotypes or linkage phase from individuals who are multiply heterozygous in a given genomic region. The method is based on single-molecule dilution (SMD) of genomic template and amplification via biphasic polymerase chain reaction (booster PCR). We have verified the feasibility of the SMD method for a highly polymorphic region within the beta-globin cluster by analysis of triply heterozygous individuals of known haplotype. This approach should be useful in many studies in population or evolutionary genetics and in a variety of clinical settings.<\/jats:p>","DOI":"10.1073\/pnas.87.16.6296","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:24:44Z","timestamp":1149060284000},"page":"6296-6300","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":84,"title":["Haplotype of multiple polymorphisms resolved by enzymatic amplification of single DNA molecules."],"prefix":"10.1073","volume":"87","author":[{"given":"G","family":"Ruano","sequence":"first","affiliation":[{"name":"Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K K","family":"Kidd","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J C","family":"Stephens","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1990,8]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.87.16.6296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:24:48Z","timestamp":1649856288000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.87.16.6296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8]]},"references-count":0,"journal-issue":{"issue":"16","published-print":{"date-parts":[[1990,8]]}},"alternative-id":["10.1073\/pnas.87.16.6296"],"URL":"https:\/\/doi.org\/10.1073\/pnas.87.16.6296","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,8]]},"assertion":[{"value":"1990-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}