{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:43:07Z","timestamp":1791236587962,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1990,3]]},"abstract":"<jats:p>Anti-Ku (p70\/p80) autoantibodies in patients with scleroderma-polymyositis overlap syndrome recognize a 70-kDa\/80-kDa protein heterodimer which binds to terminal regions of double-stranded DNA. In the present study, we isolated full-length cDNAs that encode the 80-kDa Ku subunit. Initial screening of a human spleen cDNA library with anti-Ku antibodies yielded a cDNA of 1.0 kilobase (kb) (termed K71) encoding a portion of the 80-kDa Ku polypeptide (identification based on immunological criteria). In RNA blots, this cDNA hybridized with two mRNAs of 3.4 and 2.6 kb. In rescreening of a cDNA library constructed from simian virus 40-transformed human fibroblast mRNA with the K71 cDNA as a hybridization probe, three positive clones were isolated, and that bearing the longest insert (termed Ku80-6) was selected for further characterization. In vitro transcription and translation experiments produced an immunoprecipitable polypeptide which comigrated with the 80-kDa Ku subunit. The Ku80-6 cDNA proved to be 3304 nucleotides in length, with an additional poly(A) tail, closely approximating the size of the larger mRNA. It contains a single long open reading frame encoding 732 amino acids (Mr = 82,713). The putative polypeptide has a high content of acidic amino acids and a region with periodic repeat of leucine in every seventh position which may form the \"leucine zipper\" structure. In genomic DNA blots, probes derived from the opposite ends of cDNA Ku80-6 hybridized with several nonoverlapping restriction fragments from human leukocyte DNA, indicating that the gene encoding the 80-kDa Ku polypeptide is divided into several exons by intervening sequences.<\/jats:p>","DOI":"10.1073\/pnas.87.5.1777","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:35:50Z","timestamp":1149060950000},"page":"1777-1781","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":107,"title":["Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70\/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome."],"prefix":"10.1073","volume":"87","author":[{"given":"T","family":"Mimori","sequence":"first","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Ohosone","sequence":"additional","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Hama","sequence":"additional","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Suwa","sequence":"additional","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Akizuki","sequence":"additional","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Homma","sequence":"additional","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A J","family":"Griffith","sequence":"additional","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J A","family":"Hardin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Keio University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1990,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.87.5.1777","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:07:44Z","timestamp":1649855264000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.87.5.1777"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1990,3]]}},"alternative-id":["10.1073\/pnas.87.5.1777"],"URL":"https:\/\/doi.org\/10.1073\/pnas.87.5.1777","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3]]},"assertion":[{"value":"1990-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}