{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:24:04Z","timestamp":1781105044454,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:p>Iodinated recombinant human nerve growth factor (125I-rhNGF) stimulated neurite formation in PC12 cell cultures with a half-maximal potency of 35-49 pg\/ml, compared with 39-52 pg\/ml for rhNGF. In quantitative ligand autoradiography, the in vitro equilibrium binding of 125I-rhNGF to brain sections showed a 10-fold regional variation in density and was saturable, reversible, and specifically displaced by up to 74% with rhNGF or murine NGF (muNGF). At equilibrium, 125I-rhNGF bound to these sites with high affinity (Kd 52-85 pM) and low capacity (Bmax less than or equal to 13.2 fmol\/mg of protein). Calculation of 125I-rhNGF binding affinity by kinetic methods gave average Kd values of 24 and 31 pM. Computer-generated maps revealed binding in brain regions not identified previously with 125I-muNGF, including hippocampus; dentate gyrus; amygdala; paraventricular thalamus; frontal, parietal, occipital, and cingulate cortices; nucleus accumbens; olfactory tubercle; subiculum; pineal gland; and medial geniculate nucleus. NGF binding sites were distributed in a 2-fold increasing medial-lateral gradient in the caudate-putamen and a 2-fold lateral-medial gradient in the nucleus accumbens. 125I-rhNGF binding sites were also found in most areas labeled by 125I-muNGF, including the interpedunucular nucleus, cerebellum, forebrain cholinergic nuclei, caudoventral caudate-putamen, and trigeminal nerve nucleus. 125I-rhNGF binding sites were absent from areas replete with low-affinity NGF binding sites, including circumventricular organs, myelinated fiber bundles, and choroid plexus. The present analysis provides an anatomical differentiation of high-affinity 125I-rhNGF binding sites and greatly expands the number of brain structures that may respond to endogenous NGF or exogenously administered rhNGF.<\/jats:p>","DOI":"10.1073\/pnas.88.1.281","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T04:01:25Z","timestamp":1149048085000},"page":"281-285","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["Recombinant human nerve growth factor is biologically active and labels novel high-affinity binding sites in rat brain."],"prefix":"10.1073","volume":"88","author":[{"given":"C A","family":"Altar","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L E","family":"Burton","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G L","family":"Bennett","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Dugich-Djordjevic","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1991,1]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.88.1.281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:16:21Z","timestamp":1649855781000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.88.1.281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1073\/pnas.88.1.281"],"URL":"https:\/\/doi.org\/10.1073\/pnas.88.1.281","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,1]]},"assertion":[{"value":"1991-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}