{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T02:21:11Z","timestamp":1782872471030,"version":"3.54.5"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"18","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1991,9,15]]},"abstract":"<jats:p>A 13,863-base-pair (bp) putative centromeric DNA fragment has been isolated from a human genomic library by using a probe obtained from metaphase chromosomes of human colon carcinoma cells. The abundance of this DNA was estimated to be 16-32 copies per genome. Cotransfection of mouse cells with this sequence and a selectable marker gene (aminoglycoside 3'-phosphotransferase type II, APH-II) resulted in a transformed cell line carrying an additional centromere in a dicentric chromosome. This centromere was capable of binding an anti-centromere antibody. In situ hybridization demonstrated that the human DNA sequence as well as the APH-II gene and vector DNA sequences were located only in the additional centromere of the dicentric chromosome. The extra centromere separated from the dicentric chromosome, forming a stable minichromosome. This functional centromere linked to a dominant selectable marker may be a step toward the construction of an artificial mammalian chromosome.<\/jats:p>","DOI":"10.1073\/pnas.88.18.8106","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:45:05Z","timestamp":1149061505000},"page":"8106-8110","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":32,"title":["Centromere formation in mouse cells cotransformed with human DNA and a dominant marker gene."],"prefix":"10.1073","volume":"88","author":[{"given":"G","family":"Hadlaczky","sequence":"first","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Praznovszky","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Cserp\u00e1n","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Keres\u00f6","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"P\u00e9terfy","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Kelemen","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Atalay","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Szeles","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Szelei","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Tubak","sequence":"additional","affiliation":[{"name":"Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1991,9,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.88.18.8106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:08:00Z","timestamp":1649855280000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.88.18.8106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,15]]},"references-count":0,"journal-issue":{"issue":"18","published-print":{"date-parts":[[1991,9,15]]}},"alternative-id":["10.1073\/pnas.88.18.8106"],"URL":"https:\/\/doi.org\/10.1073\/pnas.88.18.8106","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9,15]]},"assertion":[{"value":"1991-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}