{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:04:39Z","timestamp":1791331479683,"version":"4.3.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1991,10,15]]},"abstract":"<jats:p>A human cDNA coding for a protein related to the vascular permeability factor (VPF) was isolated from a term placenta cDNA library; we therefore named its product placenta growth factor (PlGF). PlGF is a 149-amino-acid-long protein and is highly homologous (53% identity) to the platelet-derived growth factor-like region of human VPF. Computer analyses reveal a putative signal peptide and two probable N-glycosylation sites in the PlGF protein, one of which is also conserved in human VPF. By using N-glycosidase F, tunicamycin, and specific antibodies produced in both chicken and rabbit, we demonstrate that PlGF, derived from transfected COS-1 cells, is actually N-glycosylated and secreted into the medium. In addition, PlGF, like VPF, proves to be a dimeric protein. Finally, a conditioned medium from COS-1 cells containing PlGF is capable of stimulating specifically the growth of CPA, a line of endothelial cells, in vitro.<\/jats:p>","DOI":"10.1073\/pnas.88.20.9267","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:47:54Z","timestamp":1149061674000},"page":"9267-9271","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":755,"title":["Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor."],"prefix":"10.1073","volume":"88","author":[{"given":"D","family":"Maglione","sequence":"first","affiliation":[{"name":"International Institute of Genetics and Biophysics, Consiglio Nazionale della Ricerce, Naples, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Guerriero","sequence":"additional","affiliation":[{"name":"International Institute of Genetics and Biophysics, Consiglio Nazionale della Ricerce, Naples, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Viglietto","sequence":"additional","affiliation":[{"name":"International Institute of Genetics and Biophysics, Consiglio Nazionale della Ricerce, Naples, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Delli-Bovi","sequence":"additional","affiliation":[{"name":"International Institute of Genetics and Biophysics, Consiglio Nazionale della Ricerce, Naples, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M G","family":"Persico","sequence":"additional","affiliation":[{"name":"International Institute of Genetics and Biophysics, Consiglio Nazionale della Ricerce, Naples, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1991,10,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.88.20.9267","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:20:40Z","timestamp":1649856040000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.88.20.9267"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,10,15]]},"references-count":0,"journal-issue":{"issue":"20","published-print":{"date-parts":[[1991,10,15]]}},"alternative-id":["10.1073\/pnas.88.20.9267"],"URL":"https:\/\/doi.org\/10.1073\/pnas.88.20.9267","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,10,15]]},"assertion":[{"value":"1991-10-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}