{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:39:02Z","timestamp":1790861942836,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"21","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1991,11]]},"abstract":"<jats:p>Organ-specific adhesion molecules expressed by vascular endothelial cells have been implicated in the arrest of blood-borne cancer cells in selective, secondary sites. A lung-specific endothelial cell adhesion molecule (Lu-ECAM-1) localized on endothelia of distinct branches of lung blood vessels has been purified by immunoaffinity chromatography from detergent extracts of lung matrix-modulated endothelial cells using monoclonal antibody (mAb) 6D3. It has a molecular mass of 90 kDa and promotes the selective attachment of lung-metastatic B16 melanoma cells. Corresponding with their metastatic performance, B16-F10 tumor cells selected for higher lung colonization bind to Lu-ECAM-1 in significantly higher numbers than their low lung metastatic counterpart B16-F0. Binding of B16-F0 and B16-F10 is reduced with mAb 6D3 to slightly lower levels than B16-F0 bound to Lu-ECAM-1. mAb 6D3 injected into C57BL\/6 mice 1 hr prior to an i.v. challenge with B16-F10 causes a 90% reduction in the number of lung colonies compared with animals injected with control mAb (6D8 or 3C6). Lu-ECAM-1 neither binds nor effects metastasis of other lung-colonizing tumor cells (e.g., KLN205). Thus, site-specific metastasis of tumor cells is regulated by similar mechanisms as the homing of lymphocytes--namely, by the ability of blood-borne cancer cells to recognize and adhere to distinct endothelial cell adhesion molecules.<\/jats:p>","DOI":"10.1073\/pnas.88.21.9568","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:47:58Z","timestamp":1149061678000},"page":"9568-9572","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":94,"title":["Mediation of lung metastasis of murine melanomas by a lung-specific endothelial cell adhesion molecule."],"prefix":"10.1073","volume":"88","author":[{"given":"D Z","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pathology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C F","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B U","family":"Pauli","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1991,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.88.21.9568","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:20:25Z","timestamp":1649856025000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.88.21.9568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,11]]},"references-count":0,"journal-issue":{"issue":"21","published-print":{"date-parts":[[1991,11]]}},"alternative-id":["10.1073\/pnas.88.21.9568"],"URL":"https:\/\/doi.org\/10.1073\/pnas.88.21.9568","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,11]]},"assertion":[{"value":"1991-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}