{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:41:09Z","timestamp":1781102469692,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1991,12,15]]},"abstract":"<jats:p>Cytotoxic T lymphocyte (CTL)-mediated cytolysis is induced via the interaction of the specific T-cell antigen receptor and the peptidic viral antigen associated with the major histocompatibility complex class I antigen. Here we demonstrate in vitro that lymphocytic choriomeningitis virus (LCMV) can escape the cytotoxic activity of LCMV-specific cloned CTLs by single amino acid changes within the recognized T-cell epitope defined by residues 275-289 of the LCMV glycoprotein [LCMV-GP-(275-289)]. LCMV-infected fibroblasts at a multiplicity of infection of 10(-3) exposed to virus-specific CTL at an effector-to-target cell ratio of 4:1 4 hr after infection was optimal for virus mutant selection. The selections were carried out with three LCMV-GP-(275-289)-specific CTL clones expressing T-cell antigen receptors containing the identical variable gene segments V alpha 4 and V beta 10 but different junctional regions; selection was also possible with LCMV-GP-(275-289)-specific cytotoxic polyclonal T cells. The most common escape mutation was an amino acid change of asparagine (AAT) to aspartic acid (GAT) at position 280; an additional mutation was glycine (GGT) to aspartic acid (GAT) at position 282. The results presented show that relevant point mutations within the T-cell epitope of LCMV-GP-(275-289) occur frequently and that they are selectable in vitro by CTLs.<\/jats:p>","DOI":"10.1073\/pnas.88.24.11047","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:52:00Z","timestamp":1149061920000},"page":"11047-11051","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":67,"title":["In vitro selection of lymphocytic choriomeningitis virus escape mutants by cytotoxic T lymphocytes."],"prefix":"10.1073","volume":"88","author":[{"given":"T","family":"Aebischer","sequence":"first","affiliation":[{"name":"Institute of Pathology, University Hospital, Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Moskophidis","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital, Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U H","family":"Rohrer","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital, Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Zinkernagel","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital, Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Hengartner","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital, Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1991,12,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.88.24.11047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:24:14Z","timestamp":1649856254000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.88.24.11047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12,15]]},"references-count":0,"journal-issue":{"issue":"24","published-print":{"date-parts":[[1991,12,15]]}},"alternative-id":["10.1073\/pnas.88.24.11047"],"URL":"https:\/\/doi.org\/10.1073\/pnas.88.24.11047","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,12,15]]},"assertion":[{"value":"1991-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}