{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:42:12Z","timestamp":1781109732457,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:p>Endothelial cells have been widely thought to be unresponsive to platelet-derived growth factor (PDGF, a major growth factor released from stimulated platelets at the sites of vascular insults) and devoid of PDGF receptors. Nevertheless, in examining the growth-factor responses of microvascular endothelial cells isolated from human omental adipose tissue, we were surprised to detect PDGF-induced tyrosine phosphorylation of a 180-kDa glycoprotein, subsequently identified as the cellular receptor for PDGF by specific immunoprecipitation. Scatchard analysis of 125I-labeled PDGF binding to human microvascular endothelial cells revealed 30,000 PDGF receptors per cell with a Kd of 0.14 nM. PDGF stimulated tyrosine phosphorylation of PDGF receptors and other cellular proteins in a dose- and time-dependent manner, with half-maximal receptor phosphorylation occurring at 0.3 nM recombinant human PDGF (B chain) and a less than or equal to 1-min exposure to PDGF. Normal cellular consequences of receptor activation were also observed, including tyrosine phosphorylation of a 42-kDa protein and serine phosphorylation of ribosomal protein S6. Furthermore, PDGF was mitogenic for these cells. Microvascular endothelial cells play a central role in neovascularization required for wound healing and solid tumor growth. Thus, the discovery of functional PDGF receptors on human microvascular endothelial cells suggests a direct role for PDGF in this process.<\/jats:p>","DOI":"10.1073\/pnas.88.5.2021","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:55:03Z","timestamp":1149062103000},"page":"2021-2025","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":95,"title":["Human microvascular endothelial cells express receptors for platelet-derived growth factor."],"prefix":"10.1073","volume":"88","author":[{"given":"J G","family":"Beitz","sequence":"first","affiliation":[{"name":"Department of Medicine, Roger Williams General Hospital, Providence, RI."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I S","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medicine, Roger Williams General Hospital, Providence, RI."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Calabresi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Roger Williams General Hospital, Providence, RI."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"A R","family":"Frackelton","sequence":"additional","affiliation":[{"name":"Department of Medicine, Roger Williams General Hospital, Providence, RI."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1991,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.88.5.2021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:16:09Z","timestamp":1649855769000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.88.5.2021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,3]]}},"alternative-id":["10.1073\/pnas.88.5.2021"],"URL":"https:\/\/doi.org\/10.1073\/pnas.88.5.2021","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,3]]},"assertion":[{"value":"1991-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}