{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:34:21Z","timestamp":1781105661136,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,5,15]]},"abstract":"<jats:p>An enzyme-linked immunosorbent assay (ELISA) was developed by using a synthetic polypeptide (SP) whose sequence was derived from the structural region of hepatitis C virus (HCV). Results of several coded panels of sera obtained from volunteer blood donors and patients with apparent non-A, non-B hepatitis and\/or hepatitis B virus used in this ELISA were compared with those of a commercially available first-generation C-100 ELISA (using nonstructural HCV antigens), an experimental second-generation C-200\/C-22 ELISA (using both structural and nonstructural HCV antigens), and recombinant immunoblot assays RIBA-I and RIBA-II. In the majority of cases, the results obtained with the HCV-SP ELISA correlated well with those obtained by RIBA-II and C-200\/C-22 ELISA. In contrast, many samples that were repeatedly reactive in the C-100 ELISA results were nonreactive with RIBA and HCV-SP ELISA. In addition, HCV-SP detected HCV-specific antibody that appeared within a month of infection and coincided with the earliest increase in alanine aminotransferase. In summary, we have developed an ELISA based on a structural HCV synthetic polypeptide, HCV-SP, that has high specificity and sensitivity and is capable of detecting specific antibodies in the acute phase of HCV infection.<\/jats:p>","DOI":"10.1073\/pnas.89.10.4486","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:01:17Z","timestamp":1149062477000},"page":"4486-4489","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":26,"title":["Detection of acute hepatitis C virus infection by ELISA using a synthetic peptide comprising a structural epitope."],"prefix":"10.1073","volume":"89","author":[{"given":"G J","family":"Kotwal","sequence":"first","affiliation":[{"name":"Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B M","family":"Baroudy","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I K","family":"Kuramoto","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F F","family":"McDonald","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G M","family":"Schiff","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P V","family":"Holland","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J B","family":"Zeldis","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,5,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.10.4486","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:17:11Z","timestamp":1649855831000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.10.4486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,5,15]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1992,5,15]]}},"alternative-id":["10.1073\/pnas.89.10.4486"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.10.4486","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,5,15]]},"assertion":[{"value":"1992-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}