{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:53:26Z","timestamp":1781103206488,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,7]]},"abstract":"<jats:p>Nitric oxide-releasing compounds were shown to activate an ADP-ribosyltransferase activity in the cytosol of Dictyostelium discoideum. The enzyme ADP-ribosylated a cytosolic protein of approximately 41 kDa, p41. Neither cGMP nor GTP and its analogues affected this ADP-ribosylation. p41 differs from other substrates ADP-ribosylated by cholera, pertussis, or diphtheria toxins. Treatment of ADP-ribosylated p41 with snake venom phosphodiesterase released adenosine 5'-monophosphate, indicating a mono-ADP-ribose-protein linkage. This linkage was stable to neutral hydroxylamine but was sensitive to mercury ions and iodomethane, suggesting an attachment to a cysteine residue. Treatment of intact cells with nitric oxide-releasing compounds appeared to stimulate the ADP-ribosylation of p41 and this modification was reversible.<\/jats:p>","DOI":"10.1073\/pnas.89.13.5902","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:02:35Z","timestamp":1149062555000},"page":"5902-5906","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["Nitric oxide stimulates the ADP-ribosylation of a 41-kDa cytosolic protein in Dictyostelium discoideum."],"prefix":"10.1073","volume":"89","author":[{"given":"Y","family":"Tao","sequence":"first","affiliation":[{"name":"E.A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Howlett","sequence":"additional","affiliation":[{"name":"E.A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Klein","sequence":"additional","affiliation":[{"name":"E.A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.13.5902","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:11:43Z","timestamp":1649855503000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.13.5902"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7]]},"references-count":0,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1992,7]]}},"alternative-id":["10.1073\/pnas.89.13.5902"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.13.5902","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7]]},"assertion":[{"value":"1992-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}