{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:36:36Z","timestamp":1781105796354,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,8,15]]},"abstract":"<jats:p>We have examined the interaction of the microbial superantigen staphylococcal enterotoxin A (SEA) with peptides corresponding to overlapping regions of the T-cell antigen receptor beta chain variable region V beta 3. SEA is known to stimulate murine T cells bearing certain V beta elements, among them V beta 3. Five peptides were synthesized representing amino acids 1-24, 20-44, 39-60, 57-77, and 74-95 of V beta 3. We demonstrate here that soluble V beta 3-bearing beta chains can bind to a complex of SEA and major histocompatibility complex class II and that the synthetic peptide V beta 3-(57-77) blocked this interaction. The peptide V beta 3-(57-77) also inhibited SEA-induced interferon-gamma production and SEA-induced proliferation of B10.BR spleen cells. Conversely, the peptide corresponding to amino acids 57-77 of V beta 8.2, a V beta element that is not recognized by SEA, decreased staphylococcal enterotoxin C-2-induced proliferation but did not affect SEA-induced proliferation. The peptide inhibition of SEA-induced function was due at least in part to inhibition of V beta 3-bearing T-cell activity, since the percentage of T cells reactive with an anti-V beta 3 monoclonal antibody was significantly reduced by V beta 3-(57-77). These data suggest that the region of V beta 3 encompassing amino acids 57-77 is an area that displays the appropriate sequence and conformation for binding of the SEA molecule and blocking of the resultant interaction with the T-cell antigen receptor.<\/jats:p>","DOI":"10.1073\/pnas.89.16.7727","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:05:55Z","timestamp":1149062755000},"page":"7727-7731","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":22,"title":["T-cell antigen receptor binding sites for the microbial superantigen staphylococcal enterotoxin A."],"prefix":"10.1073","volume":"89","author":[{"given":"C H","family":"Pontzer","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, Gainesville 32611."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Irwin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, Gainesville 32611."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N R","family":"Gascoigne","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, Gainesville 32611."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H M","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, University of Florida, Gainesville 32611."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,8,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.16.7727","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:02:58Z","timestamp":1649854978000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.16.7727"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,8,15]]},"references-count":0,"journal-issue":{"issue":"16","published-print":{"date-parts":[[1992,8,15]]}},"alternative-id":["10.1073\/pnas.89.16.7727"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.16.7727","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,8,15]]},"assertion":[{"value":"1992-08-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}