{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:12:39Z","timestamp":1791133959041,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"22","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,11,15]]},"abstract":"<jats:p>Human immunodeficiency virus type 1 (HIV-1) infections of humans have a natural history characterized by a variable but usually slow progression to an immunodeficient state. We have described a molecular model of HIV-1 proviral latency in certain cell lines, characterized by extremely low or undetectable levels of unspliced genomic HIV-1-specific RNA but significant levels of multiply spliced HIV-1-specific RNA. We have utilized a quantitative reverse transcriptase-initiated polymerase chain reaction to measure the levels of various HIV-1 RNA species in peripheral blood mononuclear cells. The median level of multiply spliced HIV-1 RNA was dramatically higher than the median level of unspliced viral RNA in asymptomatic individuals. In addition, HIV-1 RNA patterns characterized by at least a 10-fold excess of multiply spliced to unspliced viral RNA were significantly more common in asymptomatic individuals than in patients with the acquired immunodeficiency syndrome. We suggest that asymptomatic clinical HIV-1 infection is characterized by a preponderance of HIV-1-infected peripheral blood cells blocked at an early stage of HIV-1 infection. This viral expression pattern, which we have called blocked early-stage latency, may constitute a reservoir of latently infected cells in certain HIV-1-infected persons.<\/jats:p>","DOI":"10.1073\/pnas.89.22.10663","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:12:41Z","timestamp":1149063161000},"page":"10663-10667","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":73,"title":["Blocked early-stage latency in the peripheral blood cells of certain individuals infected with human immunodeficiency virus type 1."],"prefix":"10.1073","volume":"89","author":[{"given":"T","family":"Seshamma","sequence":"first","affiliation":[{"name":"Dorrance H. Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Bagasra","sequence":"additional","affiliation":[{"name":"Dorrance H. Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Trono","sequence":"additional","affiliation":[{"name":"Dorrance H. Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Baltimore","sequence":"additional","affiliation":[{"name":"Dorrance H. Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R J","family":"Pomerantz","sequence":"additional","affiliation":[{"name":"Dorrance H. Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,11,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.22.10663","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:41:15Z","timestamp":1649857275000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.22.10663"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11,15]]},"references-count":0,"journal-issue":{"issue":"22","published-print":{"date-parts":[[1992,11,15]]}},"alternative-id":["10.1073\/pnas.89.22.10663"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.22.10663","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11,15]]},"assertion":[{"value":"1992-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}