{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:54:22Z","timestamp":1781103262805,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,12]]},"abstract":"<jats:p>The beta chain of the T-cell antigen receptor present on 20 T-cell clones isolated from a tuberculoid leprosy patient was studied by gene rearrangement and PCR analysis. These T-cell clones all responded to Mycobacterium leprae-encoded protein antigens, and 8 of them specifically recognized peptides of the mycobacterial 65-kDa heat shock polypeptide (65hsp). All T-cell clones studied were HLA-DR-restricted (DR2 or -3). In the DR3-restricted group, 7 of 10 used a beta-chain variable region V beta 5 gene family member, whereas in the DR2-restricted group, 2 of 10 T-cell clones used a V beta 5 gene segment and 5 used the V beta 18 gene segment. The deduced amino acid sequences of the beta chain from 8 T-cell clones have revealed that 3 of 4 DR3-restricted T-cell clones expressed the V beta 5.1 gene segment whereas the fourth DR3-restricted T-cell clone employed a V beta 5 family member not previously described. The V beta 5.1-positive T-cell clones all recognized the same 65hsp peptide from residues 2 to 12. The N-D-N segment (where D is diversity) of the junctional region of these T-cell clones was very similar, despite different beta-chain joining gene segments. Of the 4 DR2-restricted T-cell clones investigated, 3 used the V beta 18 gene segment and recognized the 65hsp peptide from residues 418 to 427. In conclusion, within this panel of M. leprae-reactive T-cell clones, the DR3-restricted T-cell clones mainly used a V beta 5 gene segment, whereas the DR2-restricted clones employed preferentially the V beta 18 gene segment.<\/jats:p>","DOI":"10.1073\/pnas.89.23.11244","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:13:37Z","timestamp":1149063217000},"page":"11244-11248","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":15,"title":["T-cell receptor beta-chain gene usage in the T-cell recognition of Mycobacterium leprae antigens in one tuberculoid leprosy patient."],"prefix":"10.1073","volume":"89","author":[{"given":"W C","family":"van Schooten","sequence":"first","affiliation":[{"name":"Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"van der Stoep","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J B","family":"Haanen","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Pickering","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R R","family":"de Vries","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"van den Elsen","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.23.11244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:32:32Z","timestamp":1649856752000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.23.11244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12]]},"references-count":0,"journal-issue":{"issue":"23","published-print":{"date-parts":[[1992,12]]}},"alternative-id":["10.1073\/pnas.89.23.11244"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.23.11244","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,12]]},"assertion":[{"value":"1992-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}