{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:47:46Z","timestamp":1791978466631,"version":"4.4.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,12,15]]},"abstract":"<jats:p>A taurine\/beta-alanine transporter was cloned from a mouse brain cDNA library by screening with a partial cDNA probe of the glycine transporter at low stringency. The deduced amino acid sequence predicts 590 amino acids with typical characteristics of the sodium-dependent neurotransmitter transporters such as sequence homology and membrane topography. However, the calculated isoelectric point of the taurine\/beta-alanine transporter is more acidic (pI = 5.98) than those (pI &gt; 8.0) of other cloned neurotransmitter transporters. Xenopus oocytes injected with cRNA of the cloned transporter expressed uptake activities with Km = 4.5 microM for taurine and Km = 56 microM for beta-alanine. Northern hybridization showed a single transcript of 7.5 kilobases that was highly enriched in kidney and distributed evenly in various parts of the brain. In situ hybridization showed the mRNA of the taurine\/beta-alanine transporter to be localized in the corpus callosum, striatum, and anterior commisure. Specific localization of the taurine\/beta-alanine transporter in mouse brain suggests a potential function for taurine and beta-alanine as neurotransmitters.<\/jats:p>","DOI":"10.1073\/pnas.89.24.12145","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:14:57Z","timestamp":1149063297000},"page":"12145-12149","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":222,"title":["Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain."],"prefix":"10.1073","volume":"89","author":[{"given":"Q R","family":"Liu","sequence":"first","affiliation":[{"name":"Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"L\u00f3pez-Corcuera","sequence":"additional","affiliation":[{"name":"Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Nelson","sequence":"additional","affiliation":[{"name":"Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Mandiyan","sequence":"additional","affiliation":[{"name":"Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Nelson","sequence":"additional","affiliation":[{"name":"Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,12,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.24.12145","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:31:51Z","timestamp":1649856711000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.24.12145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12,15]]},"references-count":0,"journal-issue":{"issue":"24","published-print":{"date-parts":[[1992,12,15]]}},"alternative-id":["10.1073\/pnas.89.24.12145"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.24.12145","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,12,15]]},"assertion":[{"value":"1992-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}