{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:38:32Z","timestamp":1791902312424,"version":"4.3.4"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,2,15]]},"abstract":"<jats:p>Activation of several receptors, including mu-opioid, alpha 2-adrenergic, and neuropeptide Y receptors, inhibits excitatory nonadrenergic noncholinergic (NANC) neural responses in airways, which were mediated by the release of peptides from capsaicin-sensitive sensory nerves. This raises the possibility of a common inhibitory mechanism, which may be related to an increase in K+ conductance in sensory nerves. To examine this hypothesis, we have studied whether K(+)-channel blockers inhibit the effects of neuromodulators of sensory nerves in guinea pig bronchi by using selective K(+)-channel blockers. Charybdotoxin (ChTX; 10 nM), which blocks large conductance Ca(2+)-activated K(+)-channel function, completely blocked and reversed the inhibitory effects of a mu-opioid agonist, neuropeptide Y, and an alpha 2-adrenoceptor agonist on excitatory NANC responses. Neither inhibitors of ATP-sensitive K+ channels (BRL 31660 or glibenclamide, both at 10 microM) nor an inhibitor of small conductance Ca(2+)-activated K+ channels (apamin; 0.1 microM) were effective. This suggests that ChTX-sensitive K(+)-channel activation may be a common mechanism for the prejunctional modulation of sensory nerves in airways. This may have important implications for the control of neurogenic inflammation.<\/jats:p>","DOI":"10.1073\/pnas.89.4.1325","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:16:50Z","timestamp":1149063410000},"page":"1325-1329","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":73,"title":["Calcium-activated potassium channels mediate prejunctional inhibition of peripheral sensory nerves."],"prefix":"10.1073","volume":"89","author":[{"given":"D","family":"Stretton","sequence":"first","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M G","family":"Belvisi","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P J","family":"Barnes","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,2,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.4.1325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:30:50Z","timestamp":1649856650000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.4.1325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2,15]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,2,15]]}},"alternative-id":["10.1073\/pnas.89.4.1325"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.4.1325","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,2,15]]},"assertion":[{"value":"1992-02-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}