{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:43:48Z","timestamp":1790793828252,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,2,15]]},"abstract":"<jats:p>A gene encoding steroid 18-hydroxylase (P-450C18) was isolated from a human genomic DNA library. It was identified as CYP11B2, which was previously postulated to be a pseudogene or a less active gene closely related to CYP11B1, the gene encoding steroid 11 beta-hydroxylase (P-45011 beta) [Mornet, E., Dupont, J., Vitek, A. &amp; White, P. C. (1989) J. Biol. Chem. 264, 20961-20967]. The nucleotide sequence of the promoter region of the P-450C18 gene is strikingly different from that of the P-45011 beta gene, although the sequences of their exons are 93% identical. The transient expression in Y-1 adrenal tumor cells of CAT constructs with a series of deletion mutants of promoter regions of both genes indicated that the two genes are regulated differently. P-450C18 as expressed in COS-7 cells exhibits steroid 18-hydroxylase activity to catalyze the synthesis of aldosterone and 18-oxocortisol and exhibits steroid 11 beta-hydroxylase activity as well. In contrast, P-45011 beta as expressed in the cultured cells exhibits steroid 11 beta-hydroxylase activity exclusively but fails to catalyze the synthesis of aldosterone and 18-oxocortisol. These results indicate that P-45011 beta and P-450C18 are products of two different genes and that the former participates in the synthesis of glucocorticoids whereas the latter participates in the synthesis of mineralocorticoids in humans.<\/jats:p>","DOI":"10.1073\/pnas.89.4.1458","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:16:50Z","timestamp":1149063410000},"page":"1458-1462","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":194,"title":["Role of steroid 11 beta-hydroxylase and steroid 18-hydroxylase in the biosynthesis of glucocorticoids and mineralocorticoids in humans."],"prefix":"10.1073","volume":"89","author":[{"given":"T","family":"Kawamoto","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Mitsuuchi","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Toda","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Miyahara","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Ohnishi","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Ichikawa","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Imura","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kochi Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,2,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.4.1458","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:31:14Z","timestamp":1649856674000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.4.1458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2,15]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,2,15]]}},"alternative-id":["10.1073\/pnas.89.4.1458"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.4.1458","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,2,15]]},"assertion":[{"value":"1992-02-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}