{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:39:06Z","timestamp":1781105946763,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1993,6]]},"abstract":"<jats:p>A variety of peptide-secreting endocrine cells contain a population of recycling microvesicles that share several major membrane polypeptides with neuronal synaptic vesicles (SVs). The function of these synaptic-like microvesicles (SLMVs) remains to be elucidated. It was previously suggested that SLMVs of pancreatic beta cells may store and secrete gamma-aminobutyric acid (GABA). GABA, the major nonpeptide inhibitory neurotransmitter of the central nervous system, is stored in and secreted from SVs. GABA uptake into SVs is mediated by a transporter that is driven by a vacuolar proton ATPase. GABA is also present at high concentration in the endocrine pancreas where it is selectively localized in insulin-secreting beta cells, the core cells of pancreatic islets. GABA is not present in peripheral islet cells (mantle cells), represented primarily by glucagon-secreting alpha cells. In this study, an immunoisolation procedure was used to purify SLMVs from cell lines derived from mouse beta cells and alpha cells. SLMVs obtained from the beta-cell line, but not those obtained from the alpha-cell line, displayed a GABA-transport activity dependent upon a proton electrochemical gradient generated by a vacuolar proton ATPase. These data support the hypotheses that (i) SLMVs have a secretory function similar to that of SVs and (ii) beta-cell SLMVs are involved in the secretion of GABA, which in turn may have a paracrine function on mantle cells of the islet.<\/jats:p>","DOI":"10.1073\/pnas.90.11.5317","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:23:57Z","timestamp":1149063837000},"page":"5317-5321","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":73,"title":["A gamma-aminobutyric acid transporter driven by a proton pump is present in synaptic-like microvesicles of pancreatic beta cells."],"prefix":"10.1073","volume":"90","author":[{"given":"A","family":"Thomas-Reetz","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J W","family":"Hell","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"During","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Walch-Solimena","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Jahn","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"De Camilli","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1993,6]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.90.11.5317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:37:48Z","timestamp":1649857068000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.90.11.5317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,6]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1993,6]]}},"alternative-id":["10.1073\/pnas.90.11.5317"],"URL":"https:\/\/doi.org\/10.1073\/pnas.90.11.5317","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,6]]},"assertion":[{"value":"1993-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}