{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T01:34:48Z","timestamp":1792892088194,"version":"4.5.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1993,1,15]]},"abstract":"<jats:p>We report the cloning of a Leishmania chagasi antigen gene and an evaluation of leishmaniasis patient antibody responses to the recombinant protein, rK39. rK39 contains a 39-amino acid repeat that is part of a 230-kDa protein predominant in L. chagasi tissue amastigotes. Sequence analyses showed this protein, LcKin, to be related to the kinesin superfamily of motor proteins. Southern blot analyses demonstrated LcKin-related sequences in seven species of Leishmania, with conservation of the repeat between L. chagasi and Leishmania donovani. Serological evaluation revealed that 98% (56 of 57) of Brazilian and 100% (52 of 52) of Sudanese visceral leishmaniasis patients have high antibody levels to the rK39 repeat. Detectable anti-K39 antibody was virtually absent in cutaneous and mucosal leishmaniasis patients and in individuals infected with Trypanosoma cruzi. The data show that rK39 may replace crude parasite antigens as a basis for serological diagnosis of visceral leishmaniasis.<\/jats:p>","DOI":"10.1073\/pnas.90.2.775","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:32:43Z","timestamp":1149064363000},"page":"775-779","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":292,"title":["Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American visceral leishmaniasis."],"prefix":"10.1073","volume":"90","author":[{"suffix":"Jr","given":"J M","family":"Burns","sequence":"first","affiliation":[{"name":"Seattle Biomedical Research Institute, WA 98109."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W G","family":"Shreffler","sequence":"additional","affiliation":[{"name":"Seattle Biomedical Research Institute, WA 98109."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D R","family":"Benson","sequence":"additional","affiliation":[{"name":"Seattle Biomedical Research Institute, WA 98109."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H W","family":"Ghalib","sequence":"additional","affiliation":[{"name":"Seattle Biomedical Research Institute, WA 98109."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Badaro","sequence":"additional","affiliation":[{"name":"Seattle Biomedical Research Institute, WA 98109."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S G","family":"Reed","sequence":"additional","affiliation":[{"name":"Seattle Biomedical Research Institute, WA 98109."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1993,1,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.90.2.775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:47:50Z","timestamp":1649857670000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.90.2.775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,1,15]]}},"alternative-id":["10.1073\/pnas.90.2.775"],"URL":"https:\/\/doi.org\/10.1073\/pnas.90.2.775","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1,15]]},"assertion":[{"value":"1993-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}