{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:45:49Z","timestamp":1790261149771,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1993,10,15]]},"abstract":"<jats:p>Previously, we identified a soluble salicylic acid (SA)-binding protein (SABP) in tobacco whose properties suggest that it may play a role in transmitting the SA signal during plant defense responses. This SA-binding activity has been purified 250-fold by conventional chromatography and was found to copurify with a 280-kDa protein. Monoclonal antibodies capable of immunoprecipitating the SA-binding activity also immunoprecipitated the 280-kDa protein, indicating that it was responsible for binding SA. These antibodies also recognized the 280-kDa protein in immunoblots of the partially purified SABP fraction or the crude extract. However, when the crude extract was prepared in the presence of antioxidants, only a 57-kDa protein was recognized. Since the SABP has a native molecular mass of 240 kDa, it appears that the SABP is a complex which contains a 57-kDa subunit and perhaps one or more additional proteins which are covalently crosslinked in the absence of antioxidants. The ability of a variety of phenolic compounds to compete with SA for binding to the SABP was both qualitatively and quantitatively correlated with their biological activity in inducing defense-related genes. Moreover, the inducibility of the pathogenesis-related (PR)-1 genes by SA was proportional to the abundance of the SABP in different organs. These correlations are consistent with a role for the SABP in perceiving and transducing the SA signal in plant defense.<\/jats:p>","DOI":"10.1073\/pnas.90.20.9533","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:32:55Z","timestamp":1149064375000},"page":"9533-9537","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":168,"title":["Purification and characterization of a soluble salicylic acid-binding protein from tobacco."],"prefix":"10.1073","volume":"90","author":[{"given":"Z","family":"Chen","sequence":"first","affiliation":[{"name":"Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855-0759."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J W","family":"Ricigliano","sequence":"additional","affiliation":[{"name":"Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855-0759."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D F","family":"Klessig","sequence":"additional","affiliation":[{"name":"Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855-0759."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1993,10,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.90.20.9533","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:44:06Z","timestamp":1649857446000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.90.20.9533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,10,15]]},"references-count":0,"journal-issue":{"issue":"20","published-print":{"date-parts":[[1993,10,15]]}},"alternative-id":["10.1073\/pnas.90.20.9533"],"URL":"https:\/\/doi.org\/10.1073\/pnas.90.20.9533","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,10,15]]},"assertion":[{"value":"1993-10-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}