{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:25:53Z","timestamp":1781101553817,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:p>The characteristic histopathology and major histocompatibility complex associations in juvenile rheumatoid arthritis suggest an oligoclonal antigen-specific T-cell population may be critical to pathogenesis. To test this, we analyzed the T-cell repertoire of a polyarticular HLA-DR4+ juvenile rheumatoid arthritis patient with an aggressive form of disease that required arthrocentesis of the knee joints and early replacement of both hip joints. A comparison of T-cell-receptor beta chain variable region (V beta) gene expression in peripheral blood and synovial fluid performed by semiquantitation of cDNA samples amplified by the PCR revealed overexpression of the T-cell-receptor V beta 14 gene family. To determine the nature of V beta 14 overexpression, we sequenced randomly cloned amplification products derived from two synovial fluid, two synovial tissue, and three peripheral blood samples by using a V beta 14\/beta chain constant region primer pair. Sequence data showed that the T-cell response in the synovia was oligoclonal. Of four clones found, one was present in all joints examined and persisted over time. This clone accounted for 67% and 74% of all V beta 14+ clones sequenced in two synovial fluid samples and 75% and 40% in two synovial tissue samples. This clone was also found at a lesser frequency in peripheral blood samples. Further studies provided evidence for the presence of oligoclonally expanded populations of T cells utilizing the V beta 14 T-cell receptor in 6 of 27 patients examined. In contrast to the remaining patients studied, 3 with a late onset polyarticular course who exhibited especially marked clonality were characterized by features typical of adult rheumatoid arthritis (IgM rheumatoid factor-positive and HLA-DR4+). These data suggest a role for V beta 14+ T cells in a group of juvenile rheumatoid arthritis patients.<\/jats:p>","DOI":"10.1073\/pnas.90.23.11104","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:36:22Z","timestamp":1149064582000},"page":"11104-11108","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["Dominant T-cell-receptor beta chain variable region V beta 14+ clones in juvenile rheumatoid arthritis."],"prefix":"10.1073","volume":"90","author":[{"given":"A A","family":"Grom","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229-3039."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S D","family":"Thompson","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229-3039."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Luyrink","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229-3039."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Passo","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229-3039."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229-3039."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D N","family":"Glass","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229-3039."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1993,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.90.23.11104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:26:22Z","timestamp":1649856382000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.90.23.11104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12]]},"references-count":0,"journal-issue":{"issue":"23","published-print":{"date-parts":[[1993,12]]}},"alternative-id":["10.1073\/pnas.90.23.11104"],"URL":"https:\/\/doi.org\/10.1073\/pnas.90.23.11104","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12]]},"assertion":[{"value":"1993-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}