{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:52:44Z","timestamp":1791219164339,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1993,12,15]]},"abstract":"<jats:p>Natural killer (NK) cells recognize alloantigens on normal cells. One of these alloantigens correlates with homozygosity for a dimorphism of HLA-C at positions 77-80, which is shared by a number of HLA-C alleles. A second allelic alloantigen correlates with homozygosity for the alternative HLA-C dimorphism, which is shared by the remaining HLA-C alleles. Moreover, NK1- and NK2-specific NK cell lines can be generated by mixed leukocyte cultures in which donor and stimulator are homozygous for the alternative dimorphisms at positions 77-80 of HLA-C. In the present work, the role of HLA-C in NK cell-mediated allorecognition was directly investigated by analyzing the effects produced by transfection of several HLA-C alleles on NK sensitivity of class I-deleted mutant cell lines. Transfection of cells with HLA-C alleles encoding Asn-77-Lys-80 (including HLA-Cw4, -Cw5, and -Cw6) inhibited the lysis of the targets by NK1-specific NK cells, whereas HLA-C alleles encoding Ser-77-Asn-80 (including HLA-Cw1, -Cw7, and -Cw13) protected the targets from NK2-specific NK cells. Thus, HLA-C alleles are the dominant inhibitory ligands that protect targets from lysis by these allospecific NK cells.<\/jats:p>","DOI":"10.1073\/pnas.90.24.12000","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:36:45Z","timestamp":1149064605000},"page":"12000-12004","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":343,"title":["HLA-C is the inhibitory ligand that determines dominant resistance to lysis by NK1- and NK2-specific natural killer cells."],"prefix":"10.1073","volume":"90","author":[{"given":"M","family":"Colonna","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Borsellino","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Falco","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G B","family":"Ferrara","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Strominger","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1993,12,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.90.24.12000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:26:33Z","timestamp":1649856393000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.90.24.12000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12,15]]},"references-count":0,"journal-issue":{"issue":"24","published-print":{"date-parts":[[1993,12,15]]}},"alternative-id":["10.1073\/pnas.90.24.12000"],"URL":"https:\/\/doi.org\/10.1073\/pnas.90.24.12000","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12,15]]},"assertion":[{"value":"1993-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}