{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T20:53:11Z","timestamp":1781643191410,"version":"3.54.5"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1993,4,15]]},"abstract":"<jats:p>The B-cell surface molecule CD22, when cross-linked, modulates signaling through the surface IgM (sIgM)-B-cell receptor (BCR) complex. Here we analyzed the basis of this interaction between CD22 and the human sIgM complex. After lysis of B cells or B-cell lines in digitonin, CD22 coimmunoprecipitated a kinase activity that in vitro-phosphorylated two polypeptides of 150 and 130 kDa on tyrosine residues. By immunoblot analysis with a rabbit anti-serum specific for a synthetic peptide of CD22, we found these proteins to be CD22 itself. Furthermore, the phosphorylated 150-kDa CD22 was found in the sIgM-BCR complex maintained by digitonin, along with Ig alpha\/mb-1, Ig beta\/B29, and a 75-kDa polypeptide precipitated by an antiserum specific to protein-tyrosine kinase PTK72. CD22 is likely to be an important signaling partner in the sIgM-BCR complex since it is very rapidly and strikingly phosphorylated after sIgM is cross-linked and since it contains the antigen recognition homology I (ARHI) motif, present in other antigen receptor molecules.<\/jats:p>","DOI":"10.1073\/pnas.90.8.3236","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:43:27Z","timestamp":1149065007000},"page":"3236-3240","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":128,"title":["CD22 associates with the human surface IgM-B-cell antigen receptor complex."],"prefix":"10.1073","volume":"90","author":[{"given":"C","family":"Leprince","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K E","family":"Draves","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R L","family":"Geahlen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J A","family":"Ledbetter","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E A","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1993,4,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.90.8.3236","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:28:18Z","timestamp":1649856498000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.90.8.3236"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4,15]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1993,4,15]]}},"alternative-id":["10.1073\/pnas.90.8.3236"],"URL":"https:\/\/doi.org\/10.1073\/pnas.90.8.3236","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,4,15]]},"assertion":[{"value":"1993-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}