{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:33:05Z","timestamp":1781101985707,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1993,4,15]]},"abstract":"<jats:p>The membrane currents of chicken embryo fibroblasts (CEFs) transformed by Rous sarcoma virus (RSV) were compared with the currents of their nontransformed counterparts by using the whole-cell patch-clamp technique. In nontransformed CEFs, the main membrane current is a delayed outward K+ current that is sensitive to tetraethylammonium ion but insensitive to 4-aminopyridine. This K+ current is almost independent of the intracellular Ca2+ concentration and becomes completely inactivated at positive membrane potentials with a time constant of about 10 s at +30 mV. In contrast, transformed CEFs exhibit a noninactivating K+ current that strongly depends on the intracellular Ca2+ concentration. This Ca(2+)-dependent K+ current is blocked by the scorpion toxin charybdotoxin with an IC50 value of 19 nM, whereas the K+ current of normal CEFs is insensitive to charybdotoxin (up to 300 nM). The K+ current properties of transformed CEFs were also found after microinjection of purified, enzymatically active pp60v-src into normal CEFs but not after infection of CEFs with the Rous-associated virus RAV5, which lacks the v-src oncogene. Our results suggest that the oncogene product pp60v-src modulates existing K+ channel proteins, leading to profound electrophysiological and pharmacological alterations of the K+ current properties in RSV-transformed CEFs. Furthermore, our experiments identify for the first time K+ channels as possible substrates of pp60v-src.<\/jats:p>","DOI":"10.1073\/pnas.90.8.3403","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:43:27Z","timestamp":1149065007000},"page":"3403-3407","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":28,"title":["Profound differences in potassium current properties of normal and Rous sarcoma virus-transformed chicken embryo fibroblasts."],"prefix":"10.1073","volume":"90","author":[{"given":"H","family":"Repp","sequence":"first","affiliation":[{"name":"Rudolf-Buchheim-Institut f\u00fcr Pharmakologie, Justus-Liebig-Universit\u00e4t Giessen, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Draheim","sequence":"additional","affiliation":[{"name":"Rudolf-Buchheim-Institut f\u00fcr Pharmakologie, Justus-Liebig-Universit\u00e4t Giessen, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Ruland","sequence":"additional","affiliation":[{"name":"Rudolf-Buchheim-Institut f\u00fcr Pharmakologie, Justus-Liebig-Universit\u00e4t Giessen, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Seidel","sequence":"additional","affiliation":[{"name":"Rudolf-Buchheim-Institut f\u00fcr Pharmakologie, Justus-Liebig-Universit\u00e4t Giessen, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Beise","sequence":"additional","affiliation":[{"name":"Rudolf-Buchheim-Institut f\u00fcr Pharmakologie, Justus-Liebig-Universit\u00e4t Giessen, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Presek","sequence":"additional","affiliation":[{"name":"Rudolf-Buchheim-Institut f\u00fcr Pharmakologie, Justus-Liebig-Universit\u00e4t Giessen, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Dreyer","sequence":"additional","affiliation":[{"name":"Rudolf-Buchheim-Institut f\u00fcr Pharmakologie, Justus-Liebig-Universit\u00e4t Giessen, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1993,4,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.90.8.3403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:29:20Z","timestamp":1649856560000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.90.8.3403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4,15]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1993,4,15]]}},"alternative-id":["10.1073\/pnas.90.8.3403"],"URL":"https:\/\/doi.org\/10.1073\/pnas.90.8.3403","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,4,15]]},"assertion":[{"value":"1993-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}