{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:56:46Z","timestamp":1781107006830,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,5,24]]},"abstract":"<jats:p>Overaccumulation of abnormally organized mitochondria in so-called \"ragged-red\" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial encephalomyopathies. Characteristic for the abnormal mitochondria is the occurrence of highly ordered crystalline inclusions. Immuno-electron microscopy revealed that these inclusions react heavily with specific antibodies against mitochondrial creatine kinase (Mi-CK). Image processing of selected crystalline inclusions, sectioned along the crystallographic b, c planes, resulted in an averaged picture displaying an arrangement of regular, square-shaped particles with a central cavity. The overall appearance, dimensions, and symmetry of these building blocks are very reminiscent of single isolated Mi-CK octamers. Taking these findings together, it is concluded that Mi-CK octamers indeed represent the major, if not the only, component of these mitochondrial inclusions.<\/jats:p>","DOI":"10.1073\/pnas.91.11.5089","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:47:09Z","timestamp":1149065229000},"page":"5089-5093","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":81,"title":["Mitochondrial creatine kinase: a major constituent of pathological inclusions seen in mitochondrial myopathies."],"prefix":"10.1073","volume":"91","author":[{"given":"A M","family":"Stadhouders","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P H","family":"Jap","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H P","family":"Winkler","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H M","family":"Eppenberger","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Wallimann","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,5,24]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.11.5089","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:45:40Z","timestamp":1649857540000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.11.5089"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,24]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1994,5,24]]}},"alternative-id":["10.1073\/pnas.91.11.5089"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.11.5089","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5,24]]},"assertion":[{"value":"1994-05-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}