{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:12:11Z","timestamp":1789204331452,"version":"build-2803163510"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"15","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,7,19]]},"abstract":"<jats:p>ERCC4 was previously identified in somatic cell hybrids as a human gene that corrects the nucleotide-excision-repair deficiency in mutant hamster cells. The cloning strategy for ERCC4 involved transfection of the repair-deficient hamster cell line UV41 with a human sCos-1 cosmid library derived from chromosome 16. Enhanced UV resistance was seen with one cosmid-library transformant and two secondary transformants of UV41. Cosmid clones carrying a functional ERCC4 gene were isolated from a library of a secondary transformant by selecting in Escherichia coli for expression of a linked neomycin-resistance gene that was present in the sCos-1 vector. The cosmids mapped to 16p13.13-p13.2, the location assigned to ERCC4 by using somatic cell hybrids. Upon transfection into UV41, six cosmid clones gave partial correction ranging from 30% to 64%, although all appeared to contain the complete gene. The capacity for in vitro excision of thymine dimers from a plasmid by transformant cell extracts correlated qualitatively with enhanced UV resistance.<\/jats:p>","DOI":"10.1073\/pnas.91.15.6855","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:51:38Z","timestamp":1149065498000},"page":"6855-6859","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":25,"title":["Molecular cloning of the human nucleotide-excision-repair gene ERCC4."],"prefix":"10.1073","volume":"91","author":[{"given":"L H","family":"Thompson","sequence":"first","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K W","family":"Brookman","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C A","family":"Weber","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E P","family":"Salazar","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J T","family":"Reardon","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Sancar","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Deng","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Siciliano","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,7,19]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.15.6855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:39:03Z","timestamp":1649857143000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.15.6855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,7,19]]},"references-count":0,"journal-issue":{"issue":"15","published-print":{"date-parts":[[1994,7,19]]}},"alternative-id":["10.1073\/pnas.91.15.6855"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.15.6855","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,7,19]]},"assertion":[{"value":"1994-07-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}