{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:28:36Z","timestamp":1781108916858,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1994,9,27]]},"abstract":"<jats:p>We have cloned a mouse cDNA that is a member of the Krox gene family and encodes a protein we have named c-Krox. The c-Krox protein contains three zinc fingers of the Cys2His2 type. c-Krox binds specifically to a guanine-rich cis-acting element present twice in the promoter element of the mouse alpha 1(I) collagen gene. Study of c-Krox gene expression shows that c-Krox is markedly enriched in skin, one of the two major sites of type I collagen synthesis, but is absent in bone, the other main type I collagen-producing tissue, indicating that type I collagen gene expression is differentially regulated in skin and bone. DNA transfection experiments in mouse NIH 3T3 fibroblasts, cells that express the c-Krox gene, or in Drosophila S2 cells, which do not express c-Krox, reveal that c-Krox can activate transcription of a reporter gene linked to several copies of its binding site in the alpha 1(I) collagen promoter. Thus, c-Krox is only the second member of the Krox family for which a target gene has been identified. The selective spatial pattern of expression of its mRNA and its transcription activation ability suggest that c-Krox may be an important regulator of type I collagen skin specific expression in physiologic conditions and in fibrotic diseases such as scleroderma.<\/jats:p>","DOI":"10.1073\/pnas.91.20.9372","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:56:09Z","timestamp":1149065769000},"page":"9372-9376","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":75,"title":["c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin."],"prefix":"10.1073","volume":"91","author":[{"given":"P","family":"Gal\u00e9ra","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Musso","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Ducy","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Karsenty","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1994,9,27]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.91.20.9372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:04:16Z","timestamp":1649858656000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.91.20.9372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9,27]]},"references-count":0,"journal-issue":{"issue":"20","published-print":{"date-parts":[[1994,9,27]]}},"alternative-id":["10.1073\/pnas.91.20.9372"],"URL":"https:\/\/doi.org\/10.1073\/pnas.91.20.9372","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,9,27]]},"assertion":[{"value":"1994-09-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}